Table 1 Summary of merged sequencing data in a comparison between dCATCH-Seq and CATCH-Seq
From: dCATCH-Seq: improved sequencing of large continuous genomic targets with double-hybridization
Experiment | Total reads | Aligned reads | Mean PCR duplicates (%) | Aligned reads on target | On-target rate (%) | Uniquely aligned readsa | Uniquely aligned reads on targeta | On-target rate (%)a | Mean coverage on target |
|---|---|---|---|---|---|---|---|---|---|
dCATCH-Seq CATCH-Seq | 853,358 841,154 | 414,965 802,931 | 47.4 2.8 | 382,625 291,808 | 92.21 36.34 | 406,331 762,445 | 379,084 289,558 | 93.29 37.98 | 145.98 110.18 |