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Fig. 3 | BMC Genomics

Fig. 3

From: Identification of 15 candidate structured noncoding RNA motifs in fungi by comparative genomics

Fig. 3

Sequence, structure and activity of the bimolecular HDV construct from Aspergillus niger. a Sequence and secondary structure model for an HDV variant ribozyme from A. niger. Annotations are as described for Fig. 2c. b PAGE separation of 5ˊ 32P-labeled substrate RNA after partial digestion with RNase T1 (T1), partial digestion with alkali (–OH), or incubation with 100 nM of the ribozyme strand (R) of the A. niger bimolecular construct under permissive reaction conditions (See Materials and methods). NR designates no reaction. Bands corresponding to the substrate (S), 5ˊ cleavage product (5ˊ Clv) and various products generated by RNase T1 cleavage after G residues are also identified. c Mass spectrum analysis of the cleavage products. Peaks that are close to calculated masses of the 9-nucleotide 5´ Clv and 10-nucleotide 3´ Clv products are noted on the graph. The calculated (calc.) and observed (obs.) masses for the cleavage products are listed

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