Fig. 8
Hierarchical clustering analysis of quantitative RT-PCR data for photorespiratory and nitrogen metabolism genes. Transcript levels were determined at the indicated time periods after the transfer of WT and Ljgln2–2 mutant plants from CO2-enriched (time zero) to normal CO2 conditions. Relative transcript levels of WT plants under CO2-enriched atmosphere were taken as 1. Data are presented as the log2 of the difference of transcript levels between WT and Ljgln2–2. The genes considered and their corresponding accession numbers according to the Kazusa database are: asparagine synthetase (LjASN1, Lj2g3v2291670.1; LjASN2, Lj0g3v0295349.1; LjASN3, Lj0g3v0361789.1); asparaginase (LjNSE1, Lj5g3v0296030.1; LjNSE2, Lj4g3v1736160.1; LjNSE3, Lj0g3v0303539.1); serine hydroxymethyltransferase (LjSHMT1, Lj2g3v1467880.1); ferredoxin-dependent GOGAT (LjGLU1, Lj1g3v4154900.1); NADH-GOGAT (LjGLT1, LjSGA_035611.1*; LjGLT2, LjSGA_037992.1*); serine:glyoxylate aminotransferase (LjSGAT1, Lj6g3v0937010.1; LjSGAT2, Lj2g3v3058530.1); plastidic glutamine synthetase (LjGLN2, Lj6g3v1887800.1); cytosolic glutamine synthetase (LjGLN1.1, Lj2g3v0658180.1; LjGLN1.2, Lj6g3v0410490.1; LjGLN1.3, Lj0g3v0335159.1; LjGLN1.4, LjSGA_058827.1*; LjGLN1.5, LjSGA_019428.1*); glutamate dehydrogenase (LjGDH1, Lj1g3v3975110.1; LjGDH2, Lj4g3v1212370.1; LjGDH3, Lj2g3v1988990.1; LjGDH4, Lj0g3v0102829.1); hydroxypyruvate reductase (LjHPR, Lj5g3v2242500.1); glycine decarboxylase (LjGDC-H1, Lj4g3v0654460.1; LjGDC-P1, chr5.CM0019.20.r2.m*; LjGDC-P2, chr5.LjT34K16.170.r2.m*; LjGDC-T, Lj6g3v1849480.1); glycerate kinase (LjGlyK2, Lj3g3v2247080.1); glycolate oxidase (LjGO2, Lj3g3v1048900.2); plastidic dicarboxylate transporter (LjDiT1, chr5.CM0089.610.r2.d*; LjDiT2.1, Lj6g3v2204740.1) and phosphoglycolate phosphatase (LjPglP1, Lj1g3v2842370.1; LjPglP2, Lj6g3v1708420.1). The gene accession numbers are reported according to the version 3.0 of the L. japonicus genome in the Kazusa database, except in the cases indicated with an asterisk where the version 2.5 of the genome was used