Fig. 9

Clustering analysis of transcript levels of asparagine metabolism genes and photorespiratory and N metabolism genes. The clustering analysis was carried out with the Expander software using complete linkage. The mean of the expression level of each gene in all the samples analyzed was calculated and transformed in a log₂ before clustering analysis. In the color panels, each vertical line represents a single gene, and the color of the line indicates the expression level (in a log scale) of the gene relative to a specific sample: high expression in red, low expression in green. The photorespiratory genes and genes of nitrogen metabolism present in the clustering image are: asparagine synthetase (LjASN1, LjASN2 and LjASN3); asparaginase (LjNSE1 and LjNSE3); nitrate reductase (LjNR, Lj0g3v0006719.1); nitrite reductase (LjNiR, Lj4g3v0588830.1); serine hydroxymethyltransferase (LjSHM1); ferredoxin-dependent glutamate synthase (LjGLU1); NADH-dependent glutamate synthase (LjGLT1 and LjGLT2); serine:glyoxylate aminotransferase (LjSGAT1 and LjSGAT2); plastidic glutamine synthetase (LjGLN2); cytosolic glutamine synthetase (LjGLN1.1, LjGLN1.2, LjGLN1.3 and LjGLN1.5) glutamate dehydrogenase (LjGDH1, LjGDH2, LjGDH3 and LjGDH4); hydroxypyruvate reductase (LjHPR); glycine decarboxylase (LjGDC-H1, LjGDC-P1, LjGDC-P2 and LjGDC-T); glycerate kinase (LjGlyK2); glycolate oxidase (LjGO2); plastidic dicarboxylate transporter (LjDiT1 and LjDiT2.1) and phosphoglycolate phosphatase (LjPglP1 and LjPglP2). The accession numbers of the genes mentioned previously (Fig. 8) can be found in the corresponding figure legend