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Fig. 2 | BMC Genomics

Fig. 2

From: Sequencing flow-sorted short arm of Haynaldia villosa chromosome 4V provides insights into its molecular structure and virtual gene order

Fig. 2

FISH using pHv-Gypsy1 as probe, confirming its specificity to H. villosa chromosomes. The arrow indicated the chromosomes of H. villosa. a, b, c and d showed chromosomes of T. durum-H.villosa amphiploid (AABBVV) and E, F, G and H showed chromosomes of common wheat-H. villosa disomic substitution line DS3V. a Merged image from b, c and d; (b) 4′,6-diamidino-2-phenylindole (DAPI) stained chromosomes; (c) GISH analysis using H. villosa genome DNA as probe (Green), the 14 H. villosa chromosomes were shown green; (d) FISH analysis using pHv-Gypsy1 as probe (Red), the 14 H. villosa chromosomes were shown red. e Merged image from f, g and h; (f) DAPI stained chromosomes; (g) GISH analysis using H. villosa genome DNA as probe (Green), the pair of chromosome 3 V were shown green; (h) FISH analysis using pHv-Gypsy1 as probe (Red), the pair of chromosome 3 V were shown red

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