Fig. 6From: Metabolic differentiation of surface and invasive cells of yeast colony biofilms revealed by gene expression profilingGlycogen and trehalose content in colonies. a Presence of glycogen (in brown) in 3-day-old BR-F colonies. Thin cross-sections of colonies were stained and observed using transmitted light. b Glycogen and trehalose content in aerial cells from wt, gip2Δ, pig1Δ and pig2Δ colonies. The mean of three biological and 3-4 technical replicates each is shown ± SD, ***p < 0.0001Back to article page