Fig. 10
From: miRNA alteration is an important mechanism in sugarcane response to low-temperature environment
Functional analysis of miR156 in tobacco infiltrated by Agrobacterium tumefaciens strain EHA105. a) The transcript analysis of MIR156 in infiltrated tobacco leaves; b) and c) the phenotype observed in infiltrated tobacco leaves overexpressing pCAMBIA1301 and pCAMBIA1301-MIR156 with 4 °C treatment for 24 h; d) DAB staining of tobacco leaves with 4 °C treatment for 24 h; e) expression analysis of cold response genes in infiltrated tobacco leaves with 4 °C treatment for 12 h. f) quantification of anthocyanin in tobacco leaves with 4 °C treatment for 24 h. A data point represents the mean ± SE (n = 3) (a, e, f). Lowercase letters in each graph attest significant differences within the same gene/miRNA over the treatment period, as determined by Duncan’s new multiple range test (p-value <0.05)