Skip to main content

Table 3 Microarray validation by qPCR for the HT-E2 vs. HT comparison

From: Estrogen exposure overrides the masculinizing effect of elevated temperature by a downregulation of the key genes implicated in sexual differentiation in a fish with mixed genetic and environmental sex determination

Microarray

qPCR

Gene symbol

log2 Fold change

Adjusted P -value

log2 Fold change

Adjusted P-value

amh

−2.48

0.037*

−3.18

0.011*

aqp1

2.16

0.349

0.70

0.094

col18a

−4.28

0.035*

−0.78

0.174

cyp19a1a

−1.03

0.781

−3.47

0.000***

cyp19a1b

−1.37

0.089

0.12

0.028*

dmrt1

−1.54

0.007**

−2.39

0.004**

gnrh

1.11

0.129

−1.83

0.051

igf1

5.95

0.035*

−1.73

0.149

mettl22

−1.31

0.121

−1.21

0.113

prl

−1.03

0.806

−1.94

0.018*

sox17

1.08

0.779

0.35

0.570

star

−1.87

0.051

−2.00

0.006**

tesc

−1.17

0.324

−1.88

0.017*

vasa

–

–

−0.64

0.721

wisp1

1.02

0.816

−1.51

0.045*

  1. Note: Asterisks note statistical significant differences: * = P < 0.05; ** = P < 0.01; *** = P < 0.001. N 10 individuals analyzed per gene