- Research article
- Open Access
Identification of Sertoli cell-specific transcripts in the mouse testis and the role of FSH and androgen in the control of Sertoli cell activity
- U. Soffientini†1,
- D. Rebourcet†1, 2,
- M. H. Abel3,
- S. Lee3,
- G. Hamilton4,
- P. A. Fowler5,
- L. B. Smith2, 6 and
- P. J. O’Shaughnessy1Email author
© The Author(s). 2017
- Received: 9 August 2017
- Accepted: 29 November 2017
- Published: 15 December 2017
The Sertoli cells act to induce testis differentiation and subsequent development in fetal and post-natal life which makes them key to an understanding of testis biology. As a major step towards characterisation of factors involved in Sertoli cell function we have identified Sertoli cell-specific transcripts in the mouse testis and have used the data to identify Sertoli cell-specific transcripts altered in mice lacking follicle-stimulating hormone receptors (FSHRKO) and/or androgen receptors (AR) in the Sertoli cells (SCARKO).
Adult iDTR mice were injected with busulfan to ablate the germ cells and 50 days later they were treated with diphtheria toxin (DTX) to ablate the Sertoli cells. RNAseq carried out on testes from control, busulfan-treated and busulfan + DTX-treated mice identified 701 Sertoli-specific transcripts and 4302 germ cell-specific transcripts. This data was mapped against results from microarrays using testicular mRNA from 20 day-old FSHRKO, SCARKO and FSHRKO.SCARKO mice. Results show that of the 534 Sertoli cell-specific transcripts present on the gene chips, 85% were altered in the FSHRKO mice and 94% in the SCARKO mice (mostly reduced in both cases). In the FSHRKO.SCARKO mice additive or synergistic effects were seen for most transcripts. Age-dependent studies on a selected number of Sertoli cell-specific transcripts, showed that the marked effects in the FSHRKO at 20 days had largely disappeared by adulthood although synergistic effects of FSHR and AR knockout were seen.
These studies have identified the Sertoli cell-specific transcriptome in the mouse testis and have shown that most genes in the transcriptome are FSH- and androgen-dependent at puberty although the importance of FSH diminishes towards adulthood.
- Germ cell
- Follicle-stimulating hormone
The development and function of the testis is critically dependent on the Sertoli cells. These cells initially differentiate from the coelomic epithelium, at around embryonic day 11.5 (e11.5) in the mouse, and act to induce formation of the seminiferous tubules and the fetal Leydig cell population . Post-natally, the Sertoli cells are essential for spermatogenesis and cell ablation studies have shown that the Sertoli cells are also required for development and maintenance of both the adult Leydig cells and peritubular myoid cells and for normal development of the testicular vasculature [2–4]. Some specific pathways involved in these cell-cell interactions have been identified, such as the role of DHH in Leydig cell development [5, 6] but, in most cases, the cellular and molecular mechanisms by which the Sertoli cells exert control over testis development and function are unknown. Given the central role of the Sertoli cell in testis biology, identification of these pathways has to be a high priority for current research, particularly with respect to androgen production, ageing and adult male health [7, 8]. A step towards this goal would be identification of Sertoli cell-specific transcripts – ie those testicular transcripts that are only expressed in the Sertoli cells and are likely, therefore, to be involved in Sertoli-specific functions. Previously, we have shown that cell ablation can be used to identify cell-specific transcripts  and so we have now used the recently described diphtheria toxin (DTX) model of Sertoli cell ablation  to identify Sertoli cell-specific transcripts using RNAseq. As previously , one confounding factor in identifying cell-specific transcript expression through cell ablation studies in the testis is the loss of germ cells coincident with Sertoli cell ablation . To circumvent this problem, we have used busulfan to generate a germ cell-free mouse prior to Sertoli cell ablation . This also has the added advantage that comparison between normal mice and busulfan-treated animals allows identification of germ cell-specific transcripts.
During post-natal development, and in the adult, the Sertoli cells are regulated by follicle stimulating hormone (FSH) and androgen . In the absence of FSH, or the FSH-receptor (FSHR), Sertoli cell number and germ cell number is reduced although the animals are fertile [11, 12]. In animals lacking androgen receptors (AR) specifically on the Sertoli cells (SCARKO) the Sertoli cell number is normal but spermatogenesis is blocked at meiosis and the animals are infertile . Double knockout of both the FSHR and AR in the Sertoli cells has a synergistic effect leading to a marked failure in germ cell development and spermatogenesis . There have been a number of studies which have examined global changes in transcript levels in ARKO and SCARKO mice but there has been no strong consensus about the transcripts affected in these animals . This may be partly because these studies are confounded by changes in germ cell-specific transcript levels as a result of the disruption to spermatogenesis. There have, to date, been no studies of changes in overall transcript levels in FSHRKO mice or in combined FSHRKO.SCARKO mice. Identification of Sertoli cell-specific genes affected by FSHR, AR and FSHR + AR knockout would be an important step forward in understanding how FSH and androgen regulate the Sertoli cells and, in turn, how the Sertoli cells regulate testis function. We have therefore carried out microarray studies on control, FSHRKO, SCARKO and FSHRKO.SCARKO mice and integrated the data with the cohort of Sertoli cell-specific genes identified by cell ablation. This has allowed is to identify Sertoli cell specific transcripts altered in the different mouse models.
Animals and treatments
All animal maintenance and handling followed U.K. Home Office regulations and all animal procedures were carried out under U.K. Home Office License and with the approval of local ethical review committees at the Universities of Glasgow, Edinburgh and Oxford. FSHRKO, SCARKO and FSHRKO.SCARKO mice were generated at the University of Oxford as published previously  from mice obtained originally from Prof G Verhoeven and Dr. K De Gendt (Catholic University of Leuven, Belgium) and from mouse colonies bred at the University of Oxford . Mice were killed at 1d, 5d, 20d and 8 weeks of age and testes stored in liquid N2 until used for microarray studies or qPCR. Amh-Cre;iDTR mice (referred to as iDTR mice), which carry the diphtheria toxin receptor (DTR) specifically in the Sertoli cells, were generated as described previously [2, 3] from mice originally obtained from Professor A Waisman (through the Jackson Laboratories, Maine, USA) and from Professor F Guillou (Université de Tours, France). Adult iDTR mice (>120 days) were injected once with busulfan (30 mg/Kg) or vehicle [2, 17] and 50 days later they were injected with DTX (100 ng) or vehicle. Animals were killed 7 days later and one testis from each animal was frozen and stored in liquid N2. To confirm identification of Sertoli cell transcripts from RNAseq data, mRNA was used from a cohort of adult iDTR mice described previously . These animals were injected once with DTX only (without busulfan treatment) and were killed 7d, 30d, 90d or 1 year later. Similarly, to confirm identification of germ cell transcripts from RNAseq data, mRNA was used from control mice (purchased from Harlan UK, Bicester, UK) treated once with busulfan and killed at different times up to 50 days later . Finally, control, untreated mice from a different colony (bred at the University of Glasgow)  were used to examine normal development of transcript expression.
RNA was extracted from whole testes of individual animals using RNAeasy kits with on-column DNAse treatment (Qiagen Ltd., Manchester, UK). Polyadenylated RNA Sequence libraries were constructed using the Illumina TruSeq Stranded mRNA sample prep kit (Illumina, Cambridge, UK). The library was single-end sequenced on an Illumina NextSeq 500 with the NextSeq 500/550 High Output kit version 2 for 75 sequence cycles. Sequencing depth averaged 43.7 million reads per sample (range 31.6–61.2). The sequence reads were filtered for low quality bases and contaminating adapter sequences using Cutadapt  (version 1.5). The quality-trimmed reads were aligned to the mouse genome (version GRCm38.p4) using HiSat  (version 0.1.2-beta) and mean alignment per sample was 96.9% (range 96.1–97.5%). Differential expression analysis was performed using Cuffdiff  for the Cufflinks package . The bioconductor package cummeRbund  was used to investigate the output from the Cuffdiff package. Identification of signal peptides in transcripts of interest was carried out by manual curation of relevant NCBI GenBank files (https://0-www-ncbi-nlm-nih-gov.brum.beds.ac.uk/genbank/) while secreted proteins were identified through comparison with a database downloaded from the Human Protein Atlas (https://www.proteinatlas.org/search/protein_class:Predicted%20secreted%20proteins).
Microarrays were carried out using RNA extracted from whole testes of 20-day old FSHRKO, SCARKO, FSHRKO.SCARKO and control mice. RNA from testes of individual animals was extracted using RNeasy kits (Qiagen Ltd., Manchester, UK). Samples of total RNA (8 μg) from individual animals (4 per group) were reverse transcribed and then in vitro transcribed and hybridised to mouse MOE430A arrays (Affymetrix, Santa Clara, CA, USA) according to the GeneChip expression technical manual (Affymetrix, Santa Clara, CA, USA) as previously reported . Gene transcript levels were determined from data image files using algorithms in Gene Chip Operating Software (GCOS1.2, Affymetrix). Data were normalised to testis volume and Sertoli cell number as described in Results (Additional file 1). Array data and RNAseq data were aligned using a relational database (Access, Microsoft Corp, Washington, USA) using the gene symbols to connect the databases. This allowed Sertoli cell-specific transcripts on the arrays to be identified.
Testis RNA was extracted using Trizol (Life Technologies, Paisley, UK) and levels of specific mRNA species were measured by real-time PCR. Total RNA was reverse transcribed using random hexamers and Moloney murine leukaemia virus reverse transcriptase (Superscript III, Fisher Scientific UK Ltd., Loughborough, UK) as described previously . To normalise the data, external standard (luciferase mRNA: Promega UK, Southampton, UK) was added to each testis at the start of the RNA extraction . For real-time PCR the SYBR green method was used with SYBR mastermix (Agilent Technologies, Wokingham, UK) . All primers were designed by Primer Express 2.0 (Applied Biosystems, Warrington, UK) or Primer-BLAST (https://blast.ncbi.nlm.nih.gov/Blast.cgi) using parameters previously described  and the primers used are described in Additional file 2. Transcript levels were normalised to the luciferase external standard, which generates a relative value of transcript expression per testis .
Data from the arrays were analyzed using two-factor analysis of variance (ANOVA) with each gene knock-out (FSHRKO or SCARKO) as one of the factors. Where the interaction between the factors was significant, this means that the effect of the double knockout (FSHRKO.SCARKO) was not simply an additive effect of each gene knockout alone. Where there was no significant interaction P values were taken directly from the ANOVA. Where the interaction was significant, post-hoc testing was used to calculate P values for FSHRKO and SCARKO groups. The significance of differences between groups in the RNAseq were calculated by t-test or single factor ANOVA. In all cases, differences between groups were considered significant with P < 0.05 and with the false discovery rate (FDR) = 0.05 . Real-time PCR data was analysed using single factor or two-factor ANOVA.
Effects of busulfan and DTX on the testis transcriptome
RNAseq analysis of testis transcript expression in control, busulfan-treated and busulfan + DTX-treated mice identified 25,255 transcripts which were detectable in at least one group (Additional file 3). Treatment of iDTR mice with busulfan caused a significant (P < 0.05, FDR = 0.05) change in the expression of 18,095 transcripts compared to control (6961 transcripts increased, 11,134 transcripts decreased; Additional file 4) while treatment with busulfan followed by DTX caused a significant change in levels of 5280 transcripts compared to busulfan alone (2891 transcripts increased and 2389 transcripts decreased; Additional file 4). Since treatments with busulfan and with DTX will specifically target germ cells and Sertoli cells respectively (with close to 100% efficiency) then transcripts markedly reduced following each treatment regime are highly likely to be germ cell- or Sertoli cell-specific. The only probable exceptions will be transcripts in another cell type which are highly dependent on the germ cells or Sertoli cells and which may be wrongly categorised as germ cell- or Sertoli cell-specific if their expression drops markedly after cell ablation. This is discussed further below but we would not anticipate numbers of such transcripts to be large. Transcripts in the RNAseq database that show a less-marked response to cell ablation (eg 30–70% decrease) are likely to be expressed in more than one cell type and so data in Additional file 4 should be treated with caution and should be cross-referenced to Additional file 3.
Identification of Sertoli cell-specific transcripts
From the data in Fig. 1d it is clear that different levels of stringency can be applied to the identification of Sertoli cell-specific genes. At the highest level of stringency, selecting only genes which show a greater than 5-fold increase in transcript number after busulfan followed by a 90% decrease in transcripts after DTX, identifies 114 transcripts. It is clear, however, that these cutoff limits will miss a significant portion of known Sertoli cell genes. We have, therefore, chosen less stringent criteria - more than 2 fold increase after busulfan and a 70% decrease (−3.3 fold) after DTX (and a significant effect of busulfan or busulfan + DTX) – to ensure that we identify most Sertoli cell-specific transcripts. This identifies 701 transcript species (Additional file 5) which fulfill the criteria and show a significant (P < 0.05, FDR = 0.05) reduction after DTX treatment. This list contains 495 protein-coding genes, 121 non-coding RNAs, 44 predicted pseudogenes and 41 other unclassified transcripts (Additional file 5). The disadvantage to using less stringent criteria is that transcripts decreased 70–80% after DTX are likely to be expressed at low levels in other testicular cells. Conversely, this is a list of transcripts highly enriched in the Sertoli cells which can be used to interrogate other databases and it is likely to include the overwhelming majority of Sertoli cell-specific transcripts. The data in Additional file 3 also allows investigators to apply more or less stringent criteria as necessary. Finally, the complete RNAseq dataset (Additional file 3) can be used to assess whether any gene of interest is likely to show Sertoli cell expression, even if it is expressed in more than one testicular cell type. For example, none of the platelet-derived growth factors or their receptors show any decrease in expression after DTX treatment suggesting there is little or no expression in adult mouse Sertoli cells . Similarly, inhibin/activin subunits have been reported to be expressed in several cell types in the testis  and data in Additional file 3 indicates that Inhbb is primarily a Sertoli cell product in the adult testis while Inhba is primarily expressed in somatic cells but not the Sertoli cells. As a final example, there is some uncertainty about Sox13 expression in the testis with spermatogonia, spermatocytes, Sertoli cells and Leydig cells suggested as possible sites [33–35]. Analysis of the RNAseq data shows that expression increases ~5-fold after busulfan and decreases about 30% after DTX. This would indicate that in the mouse Sox13 is not expressed in the germ cells, shows some expression in the Sertoli cells but is mostly expressed in another somatic cell type. Differences to other studies which used the rat [33, 34] may be due species-dependent variation in transcript localisation.
Functional analysis of Sertoli cell-specific transcripts
Potential protein/gene network interactions among the Sertoli cell-specific transcripts identified by the online “Search Tool for the Retrieval of Interacting Genes/Proteins” (STRING, http://www.string-db.org)  are shown in Additional file 7. Functional analysis shows significant enrichment for 46 functional pathways within the set of Sertoli cell-specific transcripts, 20 cellular component pathways and 1 KEGG pathway (Additional file 8). A number of these enriched pathways/components are associated with known Sertoli cell specific functions such as junctional signalling and cell secretion and transport. It is also notable that the list of Sertoli cell specific transcripts includes 42 encoding different receptors, including VDR, FSHR, ADORA1, NR0B1 (DAX1) and AGTR2 (Additional file 9), while 111 of the translated proteins are predicted to have signal peptides and 72 may be secreted by the cells (Additional file 9). This list of 72 secreted proteins includes 10 which do not have a signal peptide and so must be secreted through non-classical secretory pathways . Interestingly, the one KEGG pathway significantly enriched is for axon guidance while biological processes associated with neural signalling are also enriched. This is consistent with the reported ability of Sertoli cells to promote neuronal differentiation and survival in co-cultures and co-grafts  although the normal physiological importance of this is not clear beyond the high potential for the Sertoli cells to secrete trophic factors.
Identification of germ cell-specific transcripts
In the same way that Sertoli cell specific genes have been identified by Sertoli cell ablation it is possible through a comparison between control testes and busulfan-treated testes to identify germ cell-specific transcripts. Busulfan acts by inducing apoptosis in spermatogonia within 1 week of treatment  and germ cells are then gradually lost from the testis over a 25–30 day period as remaining cells mature, are released from the testis and are not replaced. At the timepoint chosen for this study (50 days after busulfan) the testes are largely devoid of germ cells although some tubules contain small pockets of regenerating germ cells . It is unlikely that these regenerating cells would have a marked effect on identification of germ cell genes given the small numbers but they may cause some genes (eg those highly expressed early in regeneration) to fall outside the criteria for selection. Selecting genes that show a significant >90% reduction in transcript levels after busulfan treatment identifies 4302 genes (Additional file 10) which includes a large number (2274) of uncharacterised genes. This is a fairly stringent approach and less stringent cutoffs can be applied to the data in Additional file 3. With the cutoff set at >90% reduction in transcript levels, it is highly likely that the vast majority of genes in Additional file 10 are of specific germ cell origin and the most highly expressed of these genes are the protamines and transition proteins. Changes in transcript levels of 4 selected germ cell-specific genes at different times after a single injection of busulfan are shown in Additional file 11. For 2 genes (Nmur1 and Chn2) expression did not change until 30 days after busulfan injection indicating that these transcripts are expressed primarily in spermatids . The remaining genes (Sofu and Osbp2) started to show a change in transcript levels around days 15–20 suggesting expression in spermatocytes.
Potential protein/gene network interactions among the germ cell-specific transcripts identified by STRING are shown in Additional file 12. Due to limitations on the number of genes/proteins that can be analysed by STRING only transcripts with average control expression >3 FPKM (2838 transcript species) were included, of which STRING recognised 1915 genes/proteins. Functional analysis shows significant enrichment for 36 functional pathways within the set of germ cell-specific transcripts, 35 cellular component pathways, 9 PFAM protein domains and 14 INTERPRO protein domains and features (Additional file 13). Most of these enriched pathways are associated with known germ cell-specific functions or features.
Comparison to previously published Sertoli cell-specific gene lists
Previous studies have described the Sertoli cell transcriptome through the use of RiboTag mice or FACS [41–43] although there is little agreement between studies on what constitutes the Sertoli cell transcriptome. To determine whether there is a consensus between Sertoli cell-specific genes identified in this study and those identified by other groups using different methods we have mapped the genes from different studies to the results of the RNAseq (Additional file 14). Sanz et al.  identified 2430 Sertoli cell specific/enriched genes by RiboTag analysis and, of these, 448 show a 70% or greater decrease after DTX in our studies and 315 would satisfy the criteria of 2-fold increase after busulfan and 70% decrease after DTX (Additional file 14). De Gendt et al.  also used a RiboTag approach and identified 508 Sertoli cell-specific genes. Of these genes, 177 show a 70% decrease after DTX in our study and 122 would satisfy the criteria of 2-fold increase after busulfan and 70% decrease after DTX (Additional file 14). Using cell sorting techniques Zimmerman et al.  identified 8459 genes expressed in the Sertoli cell although this list is not Sertoli cell-specific; rather, it is a list of all genes expressed in the Sertoli cells and so contains genes expressed in other testicular cell types (eg Actb (β-actin)). Of the 495 protein-coding genes identified above as Sertoli cell-specific through DTX ablation (Additional file 5), 317 are also identified in the Sertoli cell list reported by Zimmerman et al. . In contrast to protein-coding genes, only 18 of the non-coding/unclassified genes identified using DTX are also included in . Comparing all 4 datasets (Additional file 5, two RiboTag datasets [41, 42] and the data in ) there are 83 transcripts that appear in all (Additional file 15). This may be considered as a baseline list of Sertoli cell-specific transcripts but must be considered to be highly conservative and treated with caution as it does not include some known Sertoli cell-specific transcripts such as Rhox5, Amh, Sox9, and Gdnf.
Identification of FSH- and androgen-regulated Sertoli cell-specific transcripts
To identify Sertoli cell-specific genes regulated by FSH and androgen, microarrays were carried out using RNA extracted from 20-day old control, FSHRKO, SCARKO and FSHRKO.SCARKO mice. All data from the arrays is shown in Additional file 16 and data from Sertoli cell-specific transcripts only is shown in Additional file 17. In total, 539 Sertoli cell-specific transcripts, identified by RNAseq as above, were also present on the gene-chips used for the arrays.
The central role of the Sertoli cells in testis development and function is now established but how the Sertoli cells exert control over germ cells, Leydig cells and peritubular myoid cells remains largely unclear. Similarly, it remains uncertain how the Sertoli cells themselves are regulated by both endocrine and paracrine factors although progress has been made through use of FSHRKO and SCARKO mice, along with other mouse models. In this study, we have taken a significant step towards understanding both functional and control mechanisms associated with the Sertoli cell through identification of both the Sertoli cell-specific transcriptome and the regulation of that transcriptome by FSH and androgen.
The approach used here to identify Sertoli cell-specific transcripts has been used previously to identify the Leydig cell-specific transcriptome in the rat following EDS treatment [9, 45] and would be expected to identify the overwhelming majority of cell-specific transcripts with the same caveats described previously . Firstly, transcripts could be erroneously designated as Sertoli cell-specific if Sertoli cell ablation caused a rapid and marked decline in transcript expression in another cell type. This is the main reason why a busulfan-treated, germ cell-free model was used for this study as Sertoli cell ablation will cause rapid loss of germ cells from the testis . Nevertheless, it is possible that a small number of genes identified as Sertoli cell-specific may be Sertoli cell-dependent in another cell type. Secondly, any Sertoli cell-specific transcripts that are highly regulated by the germ cells and that are markedly down-regulated by germ cell ablation will not meet the stringency criteria set here and will be missing from the Sertoli cell-specific list. Thirdly, it is possible that Sertoli cell ablation could lead to the onset of expression of (normally) Sertoli cell-specific transcripts in another cell type. This has been described previously for a Leydig cell specific transcript following Leydig cell ablation  and the transient re-appearance of Etd and Defb36 30 days after Sertoli cell ablation (Fig. 2) suggests that this phenomenon may occur after Sertoli cell ablation. If similar ectopic expression of some transcripts occurs soon after Sertoli cell ablation it would mean that a small subset of Sertoli cell-specific transcripts may be missing from the list in Additional file 5. Finally, busulfan is a cytotoxic compound and it could have direct effects on the transcriptional regulation of somatic cells which would affect the data reported here. Mice do not normally express DTR and would not be expected to be sensitive to off-target effects of DTX but studies have shown that DTX can have receptor-independent effects including weight loss and cochlear damage . It is possible, therefore, that DTX itself could also directly affect transcription in the non-target cells of the testis.
The stringency limits used here to define Sertoli cell-specific transcripts are largely arbitrary but were chosen to ensure that known Sertoli cell genes were not excluded. There was some variation in the residual expression of the Sertoli cell transcripts after Sertoli cell ablation (eg Wt1) which is a likely indication that some of these transcipts are also expressed to a small extent in another cell type. Alternatively, we have shown that Sertoli-like cells of the tubuli recti do not express AMH-Cre and are not ablated by DTX in the iDTR model [2, 3]. For most Sertoli cell-specific transcripts these cells contribute a low background level of expression following DTX-treatment but it is possible that genes such as Wt1 are more highly expressed in the tubuli recti. Either way, Investigators can apply higher or lower stringency limits to the data in Additional file 3 as appropriate to other studies.
The Sertoli cell-specific gene list described here does not show strong similarity to other Sertoli cell-specific lists compiled using the RiboTag mouse with only 10–20% of transcripts matching [41, 42]. Perhaps surprisingly, the two RiboTag studies also show only 17% agreement between themselves. The Sertoli cell transcriptome described by Zimmermann et al.  is, however, largely consistent with data reported here. This transcriptome contains all transcript species expressed in the Sertoli cells and over 63% of the protein-coding genes identified here as Sertoli cell-specific are also included in the overall Sertoli cell transcriptome . In addition, most of the remaining 37% of non-matching, Sertoli cell-specifc, protein-coding genes are expressed at low levels in control animals in our study (expression of 141 out of a total of 184 of these genes was <1 FPKM in controls) and may have fallen below the background expression cutoff used by Zimmermann et al. . Comparing the datasets from the RiboTag mice [41, 42] with the overall Sertoli cell transcriptome  shows that 82%  and 71%  of genes identified in the RiboTag mice are expressed in the Sertoli cells, as may be expected, but the data outlined here using the iDTR mouse would suggest that the criteria for selecting Sertoli cell-specific/enriched genes may not have had adequate stringency.
Studies using a variety of techniques, including cell ablation, have highlighted the role that the Sertoli cells play in regulating testicular function in the adult [2, 3, 46–48]. These Sertoli cell actions include maintenance of germ cell and Leydig cell populations, stimulation of peritubular myoid cell activity and maintenance of the testicular vasculature . How the Sertoli cells act to orchestrate each of these activities and maintain testicular function remains largely unknown, however. The identification, here, of 72 proteins/peptides that are Sertoli cell-specific and potentially secreted by the cells now provides a valuable resource for picking apart cell-cell interactions in the adult testis. The Sertoli cells also express 42 different receptor types which illustrates the complex variety of inputs possible for each cell. These receptors include some which have been previously reported to be expressed in the Sertoli cells and to play a role in testis development and adult function such as VDR and NR0B1 (DAX1) [49, 50]. Others, such as the type 2 angiotensin II receptor (AGTR2) have not previously been shown in the Sertoli cell but mean that the cells will be responsive to angiotensin. Previous studies have reported that angiotensinogen (Agt), renin (Ren1) and angiotensin converting enzyme (Ace) transcripts are all expressed in the Leydig cells and germ cells of the testis  (and consistent with the RNAseq data (Additional file 3) reported here). The testis is likely, therefore, to have an active renin-angiotensin system and this may be another mechanism by which the Leydig cells/germ cells can regulate Sertoli cell activity. The list of receptors also includes 11 olfactory receptors although transcript levels in the Sertoli cells are generally low and the function of ectopic olfactory receptors remains uncertain in many cases . Nevertheless, the Sertoli cells also specifically express Reep1 at high levels and REEP1 functions to enhance cell surface expression of odorant receptors  suggesting they may be functional in the Sertoli cells.
Development of the FSHRKO, SCARKO and combined FSHRKO.SCARKO mouse models has considerably enhanced our understanding of the endocrine control of Sertoli cell development, particularly with respect to cell proliferation and spermatogenesis [12–14, 16]. Studies into the control of Sertoli cell transcript levels have been more difficult to interpret, however, since these knockout mouse models have marked effects on germ cell numbers and a comprehensive, Sertoli cell-specific transcriptome has been missing. This has made it challenging to identify, beyond the limited number of previously known Sertoli cell-specific transcripts, which transcript changes in each model are due to altered Sertoli cell activity and which are due to a reduction in germ cell number. Identification of the Sertoli cell-specific transcriptome has allowed us now to examine the effects of the gene-specific knockouts on Sertoli cell activity. Results show that at 20 days of age in the mouse, shortly after Sertoli cell proliferation has ceased and as the blood-testis barrier is forming [54, 55], normal expression of most Sertoli cell-specific transcripts is dependent on both androgen and FSH with the effects of the two hormones either additive or synergistic. Given that the Sertoli cells regulate germ cell development and differentiation, this Sertoli cell dependence on hormonal stimulation around 20 days is consistent with earlier studies which showed that there is significant germ cell loss in both FSHRKO and SCARKO mice at 20d . At 20 days the first wave of spermatogenesis is ongoing and it is characterised by a marked surge in germ cell apoptosis which is necessary for subsequent normal spermatogenesis . Germ cell death during the first wave is regulated by FSH [57, 58] and, possibly, by androgen  and results described here show that this regulation of spermatogenesis is associated with changes in abundance of most Sertoli cell-specific transcripts.
Data from animals lacking the AR show that in the adult androgens remain essential for normal expression of most Sertoli cell-specific transcripts and in some cases (eg Corin) the requirement for androgen becomes more acute as the animals age. This is consistent with previous data showing that androgen stimulation through the Sertoli cells is essential for spermatogenesis in the adult testis . In contrast, the role of FSH in adult testis biology has been uncertain for a number of years. Early studies showed that adult Sertoli cells in the rat or mouse are largely unresponsive to FSH  although FSH was thought to be required for the normal quantitative maintenance of germ cell production [62, 63]. Subsequent development of mice lacking FSH or its receptor (FSHR) showed that FSH is not required for fertility [12, 64] although adult germ cell numbers are reduced [14, 65], an effect which may be due to loss of FSH in the adult or to abnormal spermatogenesis during the first pubertal wave . Results reported here now show that while FSH is essential for normal Sertoli cell transcript levels at puberty, cell activity in the adult mouse appears to be largely unaffected by loss of the FSHR. In some cases, however, the effect of losing both FSHR and AR from the Sertoli cells is more marked than loss of AR alone which indicates that, in the absence of androgen, FSH can act to regulate Sertoli cell activity in the adult but cannot overcome the phenotype associated with androgen deficiency.
The Sertoli cells act to induce testis differentiation and subsequent development in fetal and post-natal life [2–6, 67] and they are essential for normal fertility in the adult. Further progress towards understanding the mechanisms which regulate these functions requires that we recognise how the different cell types in the testis are able to communicate. Identification of a robust Sertoli cell-specific transcriptome takes us closer to that goal and, combined with the total Sertoli cell transcriptome published earlier , provides us with the tools necessary to investigate regulation and function of the Sertoli cell. As an example of the potential offered by this data we have also shown, using microarray data, that most Sertoli cell-specific transcripts are FSH- and androgen-dependent during development and that they remain androgen-dependent into adulthood. This is likely to explain the observation that androgen action through the Sertoli cells is essential for spermatocyte progression through meiosis  and suggests that the effect of androgen may be a general increase in cell activity rather than activation of specific pathways.
We acknowledge the technical support provided by Mike Dodds, Laura Milne, Ana Monteiro and Sarah Smith . We also acknowledge Professor Guillou for provision of the Amh-Cre line and Professor Waisman, and the Jackson Laboratory (Maine, USA) for provision of the HBEGF (iDTR) mice.
This work was funded by BBSRC Project Grant awards (BB/J015105/1 and BB/J016209/1) (to LBS and PJOS), and a Medical Research Council Programme Grant Award (MR/N002970/1) (to LBS). The funding bodies had no role in the design of the study or in the collection, analysis and interpretation of the data.
Availability of data and materials
The datasets supporting the conclusions of this article are available at the European Nucleotide Archive (ENA), http://www.ebi.ac.uk/ena/data/search?query=PRJEB21686 and in the attached Additional files. The ENA ids and their relation to sample groups described here are shown in Additional file 18.
PJOS and LBS conceived and designed the overall study, analysed and interpreted data and drafted the manuscript. US and DR designed and carried out experimental work, acquired, analysed and interpreted data and contributed to drafting the manuscript. MHA, SL and GH acquired, analysed and interpreted data and revised the manuscript. PAF analysed and interpreted data and revised the manuscript. All authors read and approved the final manuscript.
Ethics approval and consent to participate
All animals were bred and all animal procedures carried out under U.K. Home Office License and with the approval of local ethical review committees at the Universities of Glasgow (Animal Welfare and Ethical Review Board), Edinburgh (Animal Welfare and Ethical Review Board) and Oxford (Committee on Animal Care and Ethical Review).
Consent for publication
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