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Table 1 RNA-seq statistics

From: Multiple functions of CREB-binding protein during postembryonic development: identification of target genes

A. Run Summary:-

Lane

PF* Yield (bp)

Number of PF* Clusters**

Q30%

Average Quality Score

8

21,318,423,944

318,185,432

84.88

35.60

*Passed Filter (PF). **For single read sequencing (SR), number of reads = number of clusters

B.

 

dsmalE

dsCBP

Sample

M1

M2

M3

M4

C1

C2

C3

C4

Total no. of reads

28,222,259

31,75,2000

50,71,6160

37,289,035

25,719,392

22,313,906

34,360,910

27,296,096

No. of high-quality reads

23,363,204

25,935,799

36,008,785

25,162,378

20,027,446

17,999,025

19,243,770

18,616,332

Percent reads mapped back to Tribolium genome

91.51

95.94

94.97

94.41

91.01

90.05

83.28

87.73

  1. (A) Table showing the sequencing run summary of the pooled cDNA libraries prepared using RNA isolated from larvae injected with malE and CBP dsRNA. Total 318,185,432 reads obtained from all samples with an average quality score of 35.60. (B) Summary of read statistics after Illumina Hi-seq 4000 sequencing. Total reads contain the raw number of reads obtained after de-multiplexing of total reads (318,185,432) from the sample lane (8 sample/lane) from Illumina Hi-seq 4000 sequencing. Number of high quality reads indicates the number of reads left after read trimming and filtering (Quality control step). Percentage of high quality reads unambiguously mapped to reference Tribolium genome are shown in the bottom row