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Fig. 7 | BMC Genomics

Fig. 7

From: Identification of placental nutrient transporters associated with intrauterine growth restriction and pre-eclampsia

Fig. 7

Protein expression of the amino acid transporters SLC7A7 and SLC38A5 in enriched plasma membrane fractions. Representative Western blots of SLC7A7 (a; 56 kD) and SLC38A5 (b; 51 kD) protein expression from isolated placental membrane fractions. Western blotting was performed with 30 μg membrane proteins from term (n = 4), preterm (n = 5), IUGR (n = 6), PE + IUGR (n = 6), and PE (n = 5) (Left panels). Both β-actin (43 kD) and Na+/K+ ATPase (112 kD) were used as loading controls. Relative protein expression levels from western blotting results were then calculated as the densitometric ratio of targeted transporter versus β-actin (43 kD; upper right panel) and Na+/K+ ATPase (112 kD; lower right panel). Data are expressed as median ± range. Based on Kruskal-Wallis test followed by Dunn’s multiple comparison, *, ** denote p < 0.05 and < 0.01, respectively

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