Fig. 4

Experimental validation of putative PMC CRMs. a The EpGFPII reporter construct: Of a total of 3073 PMC-enriched differential peaks identified using DNase-seq and ATAC-seq, 31 were cloned into the EpGFPII plasmid, upstream of the GFP coding sequence and the Sp-endo16 promoter, and injected into S. purpuratus eggs. b Representative images of S. purpuratus embryos injected with 7 reporter constructs, showing PMC-specific GFP expression (green fluorescence) at 48 hpf. DIC and Sp-kirrelL images show the same embryo; arrows indicate PMCs