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Fig. 1 | BMC Genomics

Fig. 1

From: UDiTaS™, a genome editing detection method for indels and genome rearrangements

Fig. 1

Schematics depicting key components, process, and applications for UDiTaS. A custom hyperactive Tn5 enzyme [14, 15] (Tn5*) with UMI, pooling barcode, and i5 sequence is assembled a. and used to tagment genomic DNA (b, top). Target regions are amplified with a sequence specific “anchor” primer, and further amplified in a second round to create the Illumina sequencing library b., bottom. UDiTaS can detect and measure many event types including: small indels, large deletions (greater than > 100 bp), and inversions; the barcode represents the Unique Molecular Index (UMI) from the tagmentation step c

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