Table 1 QTLs detected with single QTL mapping. List of QTLs with an influence on fermentation parameters, the production of extracellular metabolites and volatile secondary metabolites that were detected with single QTL mapping
| Â | Trait | QTL name | Chromosome | Start position [bp] | End position [bp] | LOD Score |
|---|---|---|---|---|---|---|
Fermentation parameters | ||||||
 8 | CO2 production rate at t80% | chr4@385.5 | IV | 1,134,839 | 1,173,812 | 4.77 |
 9 | CO2 production rate at t80% | chr4@410.0 | IV | 1,198,692 | 1,246,959 | 3.69 |
| Â | CO2 production rate at t80% | chr10@241.5 | X | 717,987 | 726,938 | 4.67 |
 20 | Fermentation time t80% | chr13@7.9 | XIII | 20,503 | 25,723 | 4.86 |
 20 | CO2 production rate at t80% | chr13@7.9 | XIII | 20,503 | 25,723 | 3.81 |
Extracellular metabolites after 80% of the fermentation | ||||||
 2 | Pyruvate yield | chr2@172.5 | II | 507,274 | 527,387 | 3.54 |
 8 | G/F ratio | chr4@386.5 | IV | 1,153,678 | 1,173,812 | 10.49 |
 9 | G/F ratio | chr4@412.2 | IV | 1,205,742 | 1,243,242 | 5.49 |
 10 | Pyruvate yield | chr7@20.4 | VII | 56,448 | 74,414 | 6.15 |
 11 | Pyruvate yield | chr7@156.9 | VII | 463,981 | 503,880 | 3.75 |
 14 | Pyruvate yield | chr9@58.7 | IX | 173,782 | 179,168 | 4.00 |
 20 | Pyruvate yield | chr13@7.9 | XIII | 20,503 | 25,723 | 4.80 |
| Â | Glycerol yield | chr13@19.3 | XIII | 52,743 | 75,040 | 4.82 |
Volatile secondary metabolites after 80% of the fermentation | ||||||
 2 | Ethyl butanoate | chr2@166.4 | II | 488,757 | 506,771 | 3.96 |
| Â | 2-methylpropanoic acid | chr3@26.1 | III | 62,518 | 111,639 | 3.96 |
| Â | Ethyl 2-methylbutanoate | chr4@71.3 | IV | 211,091 | 234,153 | 4.32 |
 3 | Ethyl 2-methylpropanoate | chr4@82.9 | IV | 216,058 | 273,735 | 3.96 |
 4 | Methionol | chr4@124.6 | IV | 365,865 | 380,035 | 4.37 |
 4 | 3-methylbutyl acetate | chr4@133.6 | IV | 376,106 | 407,166 | 4.37 |
 4 | 2-methylbutyl acetate | chr4@133.6 | IV | 397,927 | 407,166 | 5.86 |
 5 | 2-phenylethyl acetate | chr4@161.9 | IV | 455,335 | 505,548 | 6.17 |
 5 | 3-methylbutyl acetate | chr4@161.9 | IV | 455,335 | 505,548 | 4.57 |
 5 | 2-methylbutyl acetate | chr4@161.9 | IV | 478,242 | 505,548 | 6.75 |
 6 | Ethyl lactate | chr4@175.0 | IV | 521,776 | 527,398 | 3.41 |
 6 | Propanoic acid | chr4@177.5 | IV | 524,924 | 545,742 | 4.00 |
 6 | Propanol | chr4@177.5 | IV | 527,398 | 539,089 | 5.19 |
 6 | Propyl acetate | chr4@179.4 | IV | 527,398 | 560,742 | 3.61 |
 10 | Diethyl succinate | chr7@15.0 | VII | 40,689 | 56,448 | 5.44 |
 10 | Ethyl 3-methylthiopropanoate | chr7@25.5 | VII | 50,239 | 87,729 | 4.47 |
 11 | Ethyl lactate | chr7@161.6 | VII | 458,995 | 518,880 | 3.97 |
| Â | Dodecanoic acid | chr7@175.5 | VII | 494,396 | 548,880 | 4.76 |
 12 | Dodecanoic acid | chr7@195.5 | VII | 578,880 | 601,380 | 3.68 |
| Â | 2-phenylethyl acetate | chr7@294.6 | VII | 853,536 | 885,989 | 4.14 |
 17 | Octanoic acid | chr11@29.5 | XI | 77,969 | 117,578 | 3.99 |
 17 | Hexanoic acid | chr11@29.5 | XI | 82,548 | 115,238 | 6.94 |
 17 | Ethyl hexanoate | chr11@35.5 | XI | 97,410 | 115,238 | 5.43 |
 17 | Ethyl dodecanoate | chr11@41.8 | XI | 115,238 | 145,211 | 4.29 |
 17 | 2-phenylethyl acetate | chr11@42.5 | XI | 125,453 | 132,044 | 4.29 |
 18 | 2-methylpropyl acetate | chr11@123.8 | XI | 366,406 | 391,690 | 6.63 |
 18 | 2-methylpropanol | chr11@127.6 | XI | 371,345 | 400,712 | 8.44 |
 18 | 3-methylbutanol | chr11@132.7 | XI | 380,437 | 403,181 | 3.56 |
 18 | 2-methylpropanoic acid | chr11@134.4 | XI | 400,712 | 405,331 | 4.64 |
| Â | 2-methylpropanol | chr11@158.4 | XI | 470,852 | 477,578 | 3.80 |
 19 | Propyl acetate | chr12@222.6 | XII | 662,035 | 699,182 | 4.83 |
 19 | Propanol | chr12@226.9 | XII | 662,035 | 691,268 | 4.08 |
| Â | Valeric acid | chr13@102.0 | XIII | 296,288 | 312,983 | 5.87 |
 21 | Propanoic acid | chr14@41.9 | XIV | 119,900 | 146,614 | 4.67 |
 21 | Propanol | chr14@43.9 | XIV | 119,900 | 146,614 | 7.03 |
 21 | Propyl acetate | chr14@46.9 | XIV | 124,114 | 150,370 | 6.19 |
 21 | Valeric acid | chr14@48.9 | XIV | 125,823 | 150,370 | 7.72 |
| Â | Propanoic acid | chr14@58.9 | XIV | 160,420 | 185,626 | 3.83 |
| Â | Valeric acid | chr14@81.8 | XIV | 233,520 | 259,114 | 3.88 |
| Â | Ethyl 3-methylbutanoate | chr15@77.3 | XV | 212,898 | 239,482 | 3.76 |
 22 | Ethyl decanoate | chr15@139.0 | XV | 409,364 | 431,700 | 3.97 |
 22 | Ethyl octanoate | chr15@142.3 | XV | 414,810 | 438,628 | 5.02 |
 23 | 3-methylbutanoic acid | chr15@162.7 | XV | 485,607 | 511,993 | 4.29 |
 23 | 2-phenylethyl acetate | chr15@176.5 | XV | 511,993 | 545,871 | 3.56 |
| Â | Diethyl succinate | chr15@297.3 | XV | 879,033 | 901,450 | 4.46 |
| Â | 3-methylbutanoic acid | chr16@191.9 | XVI | 552,371 | 593,439 | 3.83 |
 25 | Diethyl succinate | chr16@303.9 | XVI | 899,570 | 920,003 | 4.08 |
 25 | Ethyl 3-methylbutanoate | chr16@303.9 | XVI | 904,961 | 917,224 | 3.81 |
 25 | Ethyl 2-methylbutanoate | chr16@304.1 | XVI | 904,961 | 917,224 | 3.79 |