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Fig. 6 | BMC Genomics

Fig. 6

From: Identification and characterization of microRNAs involved in ascidian larval metamorphosis

Fig. 6

Expression patterns of three miRNAs detected by whole-mount in situ hybridization. Embryos and larvae at 21, 31, and 42 hpf were hybridized with LNA probes of scrambled control (a), csa-miR-4018a (b), csa-miR-4018b (c), and csa-miR-4000f (d). The developmental stages are indicated in column headings. Hybridized signals are indicated by green arrowheads. The expression signals of csa-miR-4018a, csa-miR-4018b, and csa-miR-4000f were specifically detected in a scattered cell population at 21 and 31 hpf, and their expression signals were stronger at 31 hpf but weaker at 42 hpf. (e). Schematic representation of the genomic localization of the precursors of csa-miR-4018a, csa-miR-4018b, and csa-miR-4000f. The numbers on the blue line represent the distance between two pre-miRNAs. Scale bars represent 100 μm

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