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Fig. 7 | BMC Genomics

Fig. 7

From: Identification and characterization of microRNAs involved in ascidian larval metamorphosis

Fig. 7

Csa-miR-4000f targets the Mapk1 by interacting with its 3′-UTR. a Schema of luciferase reporter constructs carrying the 3′-UTR regions of target genes used in luciferase assays. Mapk1–3′-UTR or Mapk1–3′-UTR mutants were cloned into the downstream luciferase open reading frame of the pmirGLO dual luciferase vector. Sequence alignments of csa-miR-4000f and its predicted target gene Mapk1–3′-UTR or mutants of the Mapk1–3′-UTR are displayed in the blue line box. Asterisks (*) under letters indicate the mutant nucleotide in the Mapk1–3′-UTR mutant constructs. b Relative luciferase activity in HEK-293T cells co-transfected with pmirGLO-Mapk1–3′-UTR or pmirGLO-Mapk1–3′-UTR mutants or the empty vector pmirGLO and either csa-miR-4000f mimics or miR-control. Firefly luciferase values were normalized for transfection with Renilla luciferase activity. Student’s t-test was used to evaluate the significance of luciferase data. Asterisks (**) represented statistical significance at P < 0.01

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