Fig. 11

Verification of differential expression of eight DE genes in riboflavin deficient and rescued embryos by qRT-PCR analysis. The hepatic expression of eight DE genes was verified by independent qRT-PCR analysis of the same RNA sample used in microarray analysis. The eight genes include frizzled class receptor 2 (FZD2), feather keratin (FKER), defensin beta 9 (DEFB9), kinases (PDK4, SGK2), a phosphatase (PSPH), acetyl-CoA acetyltransferase 2 (ACAT2), and phospholipase A1 member A (PLA1A). Data were analyzed by ANOVA using the GLM procedure in SAS and mean separation using Fisher’s protected least significant difference (LSD) test. The shaded insets show the common standard error (SE) of least squares mean (LSMEAN) and significant differences (* P ≤ 0.05, ** P ≤ 0.01 or *** P ≤ 0.001) for the main effects of treatment (T) and age (A) or their interaction (T x A)