Fig. 4

A gene interaction network involving several upstream regulators and their direct target genes involved in “Cellular Growth and Proliferation” (a). Ingenuity® Up-stream Regulator Analysis predicts activation of two transcription factors, JUN proto-oncogene, AP-1 transcription factor subunit (JUN) and thyroid hormone receptor beta (THRB), and their direct target genes (b). These gene networks were identified in a combined dataset of all AR-DE genes found in the liver at e13 and e15 (see Fig. 2b). The target genes were either upregulated in riboflavin-rescued (Rf+; red-colored symbols) or expressed higher in riboflavin-deficient (Rf-; green-colored symbols) embryo livers. IPA predicts that the two up-stream regulators are “activated”, which would lead to either activation (orange arrows) or inhibition (blunt blue edges) of their respective direct target genes