Skip to main content
Fig. 10 | BMC Genomics

Fig. 10

From: Transcriptomic profiling of tall fescue in response to heat stress and improved thermotolerance by melatonin and 24-epibrassinolide

Fig. 10

qPCR analysis of heat stress induced genes in tall fescue. Eight days old seedlings grown on control MS plate were transferred to MS medium containing 20 μM melatonin (MT) or 0. 1 μM 24-epibrassinolide for 2 days, and then heat stress (38 °C) was applied for indicated time. Shoots of seedlings were collected immediately for RNA extraction. Notably, 20 genes highly induced by heat stress were selected for qPCR analysis and ten of them showed significant changes after MT or EBL treatments. The detailed information of selected genes and primers used for qPCR were listed in Additional file 1: Table S1. A: genes downregulated by both MT and EBL; B: genes upregulated by both MT and EBL; C: genes upregulated only by EBL, d, day; h, hour. Data were means ±SE of three biological replications. Bars with different letters indicate significant difference at P≤0.05 (Duncan test)

Back to article page

BMC Genomics

ISSN: 1471-2164

Contact us