Fig. 2
From: Paramutation-like features of multiple natural epialleles in tomato
H06 paramutation assessed by McrBC. McrBC digests methylated DNA, so that comparing amplification of McrBC-treated and non-treated DNA by qPCR reveals the proportion of molecules that were methylated. a H06 McrBC in M82 × IL8–3 seedling leaves. Unmethylated H06M82 (n = 12), crossed with the methylated H06IL from IL8–3 (n = 7), became methylated in most F1s (14 out of n = 15), and remained methylated in the F2 (n = 30). Back-crossing the F1 with M82 demonstrated weak paramutagenicity (BC1, n = 26, p-value = 0.03776, one-sided binomial test). Paramutation also occurred with the IL as the female parent (IL8–3 × M82, n = 1). b H06 McrBC in M82 × Micro-Tom and S.pimpinellifolium. F1 seedling leaves are fully methylated (n = 6 for each cross), like Micro-Tom and S. pimpinellifolium themselves (n = 4 and 5 respectively). (C) H06 McrBC in M82 × S. pennellii. The F1 vegetative tissue was half-methylated (n = 4) whereas the F1 pollen and the F2 leaves were fully methylated (n = 5 and 11 respectively)