Fig. 1

mRNA gene expression levels in bovine monocyte-derived macrophages as determined by reverse transcription-quantitative PCR (RT-qPCR). Genes displaying a significant difference in expression between the Streptococcus agalactiae strains ST103 and ST12, and the lipopolysaccharides (LPS) stimulated cells, respectively, compared to uninfected controls are denoted with * (P ≤ 0.05). The significant differences between responses to LPS vs. ST103, and LPS vs. ST12, respectively, are denoted with # (P ≤ 0.05). Data are presented as fold change relative to negative control, and as a mean values with SD. Tumor necrosis factor alpha (TNFα), interleukin-1 beta (IL-1β), interleukin-6 (IL-6), interleukin-8 (IL-8), interleukin-10 (IL-10) and transforming growth factor beta 1 (TGFβ1). Peptidylprolyl isomerase A (PPIA) was used as a reference gene