Upregulation of genes encoding digestive enzymes and nutrient transporters in the digestive system of broiler chickens by dietary supplementation of fiber and inclusion of coarse particle size corn

Table 11 Effect of particle size and sugar cane bagasse on expression of ileal genes at d 24^1,2

Treatments		APN	ASCT1	ATP1A1	B^oAT	CAT1	CAT2	CCK1R	EAAT3
Particle size	SB	APN	ASCT1	ATP1A1	B^oAT	CAT1	CAT2	CCK1R	EAAT3
CC	0%	1.219	1.153	1.000^b	1.000	1.053	1.077	1.240	1.204
FC	0%	1.550	1.060	1.174^ab	1.003	1.000	1.026	1.000	1.069
CC	2%	1.000	1.000	1.193^a	1.165	1.626	1.000	1.358	1.000
FC	2%	1.364	1.171	1.096^ab	1.251	2.216	1.016	1.009	1.372
Main effect
SB
0%		1.385	1.107	1.087	1.002^b	1.027^b	1.052	1.120	1.137
2%		1.182	1.085	1.145	1.208^a	1.921^a	1.008	1.184	1.187
Particle size
CC		1.110	1.077	1.097	1.082	1.340	1.038	1.299	1.102
FC		1.457	1.115	1.136	1.128	1.608	1.021	1.004	1.221
P value
SB		0.401	0.867	0.387	0.019	0.029	0.627	0.664	0.876
Particle size		0.156	0.765	0.554	0.583	0.489	0.846	0.056	0.709
SB × particle size		0.943	0.314	0.049	0.611	0.407	0.705	0.707	0.428

¹The relative expression levels of the genes of respective treatment groups are expressed as means of normalized relative quantities (NRQ). Relative quantities for individual genes are scaled to the average across all unknown samples per target gene
²Within a column, values with different superscripts are significantly different from each other at P < 0.05

ISSN: 1471-2164