Fig. 7
The reconstructed pathways of fatty acids biosynthesis in plastid and triacylglycerol biosynthesis in ER based on the de novo assembly and annotation of P. frutescens transcriptome. The icons besides the key enzymes represent the relative expression levels of their transcripts in seeds between 10DAF and 5DAF, 20DAF and 10DAF. The identified enzymes involved in fatty acid biosynthesis include α-CT, carboxyl transferase α-subunit; β-CT, carboxyl transferase β-subunit; BC, biotin carboxylase; BCCP, biotin carboxyl carrier protein; MCMT, malonyl-CoA ACP transacylase; KAS, ketoacyl-ACP synthase; KAR, ketoacyl-ACP reductase; HAD, hydroxyacyl-ACP dehydrase; EAR, enoyl-ACP reductase; SAD, stearoyl-ACP desaturase; FATA/B, acyl-ACP thioesterase A/B; FAD6, oleate desaturase (chloroplast-type); FAD7/8, linoleate desaturase (chloroplast-type). Enzymes involved in triacylglycerol synthesis are LPCAT: lysophosphatidylcholine acyltransferase; FAD2, oleate desaturase; FAD3, linoleate desaturase; GPAT, glycerol-3-phosphate acyltransferase (EC2.3.1.15);PAP, PA phosphatase (EC: 3.1.3.4); DGAT, acyl-CoA: diacylglycerol acyltransferase; PDAT, phospholipid:diacylglycerol acyltransferase (EC: 2.3.1.20); PDCT, phosphatidylcholine: diacylglycerol cholinephosphotransferase (EC:2.7.8.*)