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Table 2 Differential abundance of proteins (SILAC) and transcripts (RNA-Seq) in early and late procyclic forms

From: RNA-Seq analysis validates the use of culture-derived Trypanosoma brucei and provides new markers for mammalian and insect life-cycle stages

Up in early procyclic forms

Gene ID

Description

SILAC rep1

SILAC rep2

RNA-seq Rep1

RNA-seq Rep2

Tb927.8.5460

Calflagin (flagellar calcium-binding protein 44)

8.4

3.59

5.75

5.01

Tb927.8.2210

Pteridine reductase (PTR1)

3.07

ND

1.96

2.12

Tb927.10.3660

Aspartate aminotransferase

3.26

3.18

2.35

2.95

Tb927.10.2010

Hexokinase (HK1)

3.4

2.93

4.48

3.74

Tb927.11.4700

Prostaglandin f synthase

2.37

2.4

4.94

3.81

Tb927.9.7000

Methyltransferase domain containing protein, putative

2.61

2.93

2.54

2.24

Tb927.9.6090

PTP1-interacting protein, 39 kDa

2.74

2.28

2.40

2.10

Tb927.1.2100

Calpain-like protein 1.1 (CALP1.1)

1.09

2.03

2.70

2.60

Tb927.7.7500

Thymine-7-hydroxylase, putative (TLP7)

4.08

1.97

3.52

2.44

Up in late procyclic forms

Gene ID

Description

SILAC rep1

SILAC rep2

RNA-seq Rep1

RNA-seq Rep2

Tb927.1.3950

ALAT

2.44

1.68

3.45

0.85

Tb927.9.8720

FBPase

2.61

3.29

2.78

2.94

Tb927.7.190

Metallo-peptidase

2.77

2.58

1.40

1.67

Tb927.11.2500

Carboxypeptidase

2.46

2.72

2.13

1.79

Tb927.11.16550

Zinc finger protein, C3H1 type-like (ZC3H46)

8.78

33.24

1.0

3.03

Tb927.9.4200

Fatty acyl CoA synthetase 2 (ACS2)

2.19

2.07

1.43

1.70

Tb927.10.14140

Pyruvate kinase 1 (PYK1)

2.38

2.00

1.41

1.40

  1. Numbers denote fold changes. Two biological replicates were performed in each case. The data for SILAC are derived from Imhof et al. [6]. ND not detected