Fig. 5

HHV-6B annotation based on comparative genomics. Differences in annotation between HHV-6B Z29 and HST sequences are compared with a subset of the 119 genomes sequenced in this study. a Sanger sequencing of U91 cDNA revealed a different splice site 13-bp upstream than that which is annotated in the reference Z29 strain. The aberrant splice-site annotation in Z29 is likely due to a single base insertion found only in Z29 that alters the reading frame in the second exon. Genome sequencing of our cultured HHV-6B strain (Z29-1) confirmed the Z29-1 cDNA sequence. The reading frame depicted for Z29 is as annotated in the NCBI reference genome (NC_000898). Based on the newly discovered splice site, the Z29 U91 would contain an early stop codon while all other U91 sequences obtained in this study would continue the reading frame to the end of U91 as annotated in Z29. Several key different loci in U12 (b), U27 (c), and US52 genes (d) that alter the length of open reading frames in Z29 and HST are depicted. e A homopolymeric polymorphism in U83 changes in expected length and sequence of its open reading frame between different strains