A modified sequence capture approach allowing standard and methylation analyses of the same enriched genomic DNA sample

Table 1 Mapping statistics for the reference sequence

Sample	% of reads aligned pre-filtering	Average % coverage per ref. contig	Average depth of coverage per ref. contig	Number of ref. contigs mapped	% of ref. contigs mapped	Base-space mapped (bp)
BTF	24.2	59.6	30.7	82,873	99.6	39,868,184
BTS	24.2	59.3	30.5	82,862	99.6	39,602,695
NBTF	73.3	70.7	36.9	83,107	99.9	48,808,952
NBTS	72.8	77.9	34.5	82,999	99.7	54,641,687

Detailing the mapping output statistics for the two enriched wheat DNA samples, NBTF and BTF (non-bisulphite treated and bisulphite treated) that were taken through separate capture reactions and the two samples that were split and one bisulphite treated while the other was non-bisulphite treated after a single capture (NBTS and BTS). Mapping statistics are in relation to the 82.5 Mb mapping reference

ISSN: 1471-2164