Fig. 1
Experimental protocol used to test the effect of the duration of FSH superstimulation on gene expression of granulosa cells in cattle. Transvaginal ultrasound-guided follicle ablation was performed 5 to 8 days after ovulation to synchronize wave emergence (Day 0). Beginning on Day 0, FSH was given at 12-h intervals for 4 days (conventional FSH group) or 7 days (long FSH group; n = 6 cows per group). Cows were given PGF2α twice on Day 3 in the conventional FSH group or Day 6 in the long FSH group. Cows were ovariectomized 24 h after LH treatment