Fig. 4

Identification of P. falciparum genes contributing to cerebral malaria. a Principal component analysis performed on eight RNA-seq datasets. Components 1 (PC1) and 2 (PC2) represent 71% and 21% of total variance, respectively. Datasets of two adjacent time points tend to be located close together within the plot. b The periodic genes identified by FFT ordered by the time points of their peak expression. Expression values of each transcript were log2-scaled and centered by subtracting their mean value. c Venn diagram of the number of genes transcribed at the trophozoite stage and that of candidate genes whose proteins contain transmembrane domains. d Domain model of PIESP2 protein (upper panel) and expression signals of PIESP2 in the intraerythrocytic cycle (lower panel). TM represents transmembrane domain. Blue line represents the observed expression level of PIESP2 and red line is the fitting curve using FFT