Fig. 7

Tissue distribution and RNase R resistance of RMST and FIRRE junctions. a RMST junctions in adult tissue. b FIRRE junctions in adult tissue. Mean ΔCt values of 3 technical replicates are shown (+/− S.E.M). c RNase R sensitivity of RMST and FIRRE junctions. Left panel; –Ct values of 3 housekeeping genes in digested and mock reactions. Right panel; -ΔΔCt values of RNase R digested relative to undigested (see methods). A control linear/circRNA transcript pair (SMARCA5) is also shown. Means from 3 biological replicates are shown +/− S.E.M, except for FIRRE E2-E5 where only technical replicates were available. *p < 0.05, **p < 0.01, ***p < 0.001. d Replicate Northern blots of day 0 ES (E) and adult brain (B) RNAs, digested with RNase R (D) or undigested (U) and hybridised with i. SMARCA5 probe internal to circRNA (E15-E16), ii. SMARCA5 probe external to circRNA (E10-E13), iii. RMST probe internal to main circRNA (E6-E9), iv. RMST probe external to main circRNA (retained intron 5A-E5B), v. GAPDH probe (E3-E6). RNase R resistant transcripts are highlighted with asterisks, and approximate position of human rRNAs are shown