Fig. 6

Tlr4 gene functional study in CC042/GeniUnc. Splenic (a) and liver (b) bacterial loads at day4 p.i. in Tlr4^+/+ B6 (n = 6), Tlr4^−/− B6 (n = 2), CC042 (n = 6), (Tlr4^+/+ B6 x CC042)F1 (n = 5) and (Tlr4^−/− B6 x CC042)F1 (n = 5) mice infected with 10³ CFUs of S. Typhimurium (cumulative results from 2 distinct experiments). The TNF-alpha levels production at 90 min post-mock-PBS injection or 100 μg LPS injection (c) in Tlr4^+/+ B6 (n = 4 and 4), Tlr4^−/− B6 (n = 4 and 4) and CC042 (n = 4 and 5) mice. The values are means and error bars present standard error of mean. Mean bacterial loads (a) and (b) were compared by one-way ANOVA and Tukey HSD test. Mean TNF-alpha values (c) were compared by two-way ANOVA and Holm-Šídák test. Asterisks indicate respective P-value of P < 0.1 (*), P < 0.01 (**), P < 0.001 (***), P < 0.0001 (****)