Fig. 1

All the Ca²⁺ signal decoders (360 genes) were grouped into two clusters, together with a MAPK gene (outgroup) for phylogenetic tree construction. a Phylogenetic relationships among the Ca²⁺ signal decoders identified two monophyletic clusters. Node supporting values of near maximum-likelihood and maximum-likelihood are shown from top to bottom. b Conserved sequence insertions (shown as black bars) confirmed the phylogenetic classifications and indicated a new structured monophyly with both N-terminal kinase domain and C-terminal EF hands (shown in purple bars) that were named as the X monophyly genes. Sequences in the tree from Archaeplastida were shown in green, Amoebozoa in black, SAR in red, Opisthokonta in blue, and Excavata in purple