Fig. 8

rSOX-4 regulates expression of Tubb2b. a MA plot demonstrating differential gene expression between the rSOX-4 deleted cells and untreated S16 cells. Each dot represents a gene and red dots indicate a significant difference in expression between the two cell populations (adjusted p < 0.05). Genes with a positive or negative log2 fold change demonstrated higher or lower expression in the rSOX-4 cells line compared to the untreated S16 cells, respectively. Tubb2b and Gmnn are indicated by arrows. b Digital droplet PCR (ddPCR) was performed to validate RNA-Seq findings for Tubb2b. Data from the untreated cells are shown by the blue bars, and data from rSOX-4-deleted cells are shown by the red bars. Aldh5a1 was used as a control gene, which showed no expression changes between the two cell populations. c-f SOX10 increases Tubb2b expression in heterologous cells. MN1 cells were transfected with a construct to express GFP-SOX10, sorted into GFP-positive and GFP-negative populations, and subjected to cap analysis gene expression (CAGE). CAGE reads mapping to the Mpz (c), Mitf (d), Tubb2b (e), and Gmnn (f) loci were normalized to reads per million and are indicated in red (SOX10-positive cells) and blue (SOX10-negative cells). RefSeq-annotated transcripts at each locus in the mouse genome (mm10) are shown in black and, in each case, are transcribed from left to right