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Table 3 Genes stimulated or inhibited by dehydration within couch grass rhizomes selected for qRT-PCR analysis, sequences of the primers and amplification efficiencies

From: The dehydration stress of couch grass is associated with its lipid metabolism, the induction of transporters and the re-programming of development coordinated by ABA

IDa

AGIb

Namec

Primer sequence

Efficiency (%)d

Contig15047_at

AT5G20730.2

NPH4_F

atcctatcccctcaagaagtgcaaa

93

NPH4_R

tggtcgtaacgaggcttccaagtat

Contig1223_at

AT3G53420.2

PIP_F

agtacgtcctgagggcgagtg

92

PIP_R

cacgatccgagccatatcacactgat

Contig14720_s_at

AT1G34260.1

PI3PK_F

gagtttgtacttggcatcatcgact

95

PI3PK_R

aaccgttaggaaatacttggccatg

HVSMEf0022D18r2_s_at

AT3G22600.1

LTPG5_F

gatcgggttggcgcgcataca

92

LTPG5_R

atgcatgtcacggtacaacaaatgga

Contig10474_at

AT1G80950.1

PLGAC_F

gttgctctttcctgagggcac

108

PLGAC_R

aaaatgactggttgtactggtgctc

Contig3810_at

AT1G09350.1

WSI76_F

tacgtgcaagcacacggttgg

97

WSI76_R

acgtttcagccatgcatacgtgtacg

Contig14329_at

AT1G04870.2

PRMT10_F

ttgatgactccatctccgagagtaa

94

PRMT10_R

atccatatccataagccggtgattc

Contig10182_at

AT1G15520.1

ABC_F

tcagccctattgcatggacactcaa

94

ABC_R

gctactacccacaggaagtcgtgat

HT11N18r_s_at

AT1G78390.1

NCED_F

cttattaggcataggagatccccgg

94

NCED_R

tgaagcaagtgtgagctaactgaat

Contig1708_s_at

AT4G01985.1

DHN6_F

agcacaagaccggtggcatcct

103

DHN6_R

tccttgttaccgccggggagct

HW09B04u_at

AT4G39670.1

GLTP_F

cgttccatagctgggcaatccaga

108

GLTP_R

acagagcaatcagtttcgttgagcc

Contig2406_at

AT3G15670.1

WRAB1_F

ttgcctttgatttgatggtactcgtgt

97

WRAB1_R

gtgccacctttcgactgtcctc

Contig1718_s_at

AT3G50980.1

DHN9_F

aagacccgtgggatactgcatcgct

96

DHN9_R

gtcgccatgtgctgctggttgtc

Contig9547_at

AT2G26830.1

CEK4_F

ggcactcattcaggcaagggta

95

CEK4_R

ctcctcagtgaagaaaggaagcctt

HVSMEf0019H18r2_s_at

AT4G01470.1

TIP1;3_F

atccatgcgtcatcgccatga

96

TIP1;3_R

tgactgactcacacacagtttaccc

Contig10112_at

AT4G40060.1

HB16_F

gatcctcggacagcgactcgagcg

99

HB16_R

tgtccaggaacgacgcgccgaa

  1. aAffymetrix 22 K Barley1 GeneChip Genome Array probe set ID
  2. bA. thaliana locus identifier corresponding to individual IDs
  3. cF forward, R reverse, NPH4 Transcriptional factor B3 family protein, PIP plasma membrane intrinsic protein, aquaporin, PI3PK phosphatidylinositol-3P 5-kinase, LTPG5 glycosylphosphatidylinositol-anchored lipid transfer protein 5, PGLAC phospholipid/glycerol acyltransferase, WSI76 galactinol synthase, PRMT10 histone-arginine-N-methyltransferase, ABC ATP-binding cassette transporter, NCED 9-cis-epoxycarotenoid dioxygenase, DHN6 dehydrin DHN6, GLTP glycolipid transfer protein, WRAB1 ABA-inducible protein WRAB1, DHN9 dehydrin DHN9, CEK4 choline/ethanolamine kinase 4, TIP1;3 tonoplast intrinsic protein 1;3, HB16 homeobox protein 16
  4. dAmplification efficiency