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Table 4 Genes from which the primer sequences of candidate reference genes were derived, sequences of the primers and amplification efficiencies

From: The dehydration stress of couch grass is associated with its lipid metabolism, the induction of transporters and the re-programming of development coordinated by ABA

IDa

AGIb

Namec

Primer sequence

Efficiency (%)d

Contig1390_3_s_at

AT5G09810.1

ACT_F

tcactcagcactttccaacagatgt

105

ACT_R

gactagatgataacagcagtggagc

Contig2580_3_s_at

AT1G69410.1

IF5A_F

tttgggacccttgtgtttcctatgg

95

IF5A_R

tttctggcatacagtttgcaccgtc

Contig1008_x_at

AT5G14670.1

ADP-RF_F

tagttctctcgggatgtcgggggtc

91

ADP-RF_R

gacaaaaatgagaccctgggtgttctga

Contig21863_at

 

HSP90_R

ggacgctgtttattggctacgacga

103

HSP90_F

tccatacacacagtcgggacgtatc

Contig306_s_at

AT5G45350.2

SIGPRP_F

taccctggctcatctggtcacagtg

90

SIGPRP_R

agagatcttgtgtgctccgtaagcg

Contig149_at

AT1G13440.1

GAPDH_F

gggttcccactgtggatgtgtcagt

91

GAPDH_R

ttccctcggaagcagccttgatagc

  1. aAffymetrix 22 K Barley1 GeneChip Genome Array probe set ID
  2. bA. thaliana locus identifier corresponding to individual IDs
  3. cF forward, R reverse, ACT Actin, IF5A Translation elongation factor 5A, ADP-RF ADP-ribosylation factor, HSP90 cytosolic heat shock protein 90, SIGPRP similar to GPRP (proteins rich in glycine and proline), GAPDH glyceraldehyde-3-phosphate dehydrogenase
  4. dAmplification efficiency