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Fig. 3 | BMC Genomics

Fig. 3

From: Characterization and remediation of sample index swaps by non-redundant dual indexing on massively parallel sequencing platforms

Fig. 3

Index swapping leads to incorrect assignment of reads from fusion transcripts in cell line RNA-seq data. Counts of reads spanning fusion transcripts for 5 different gene fusions in 3 different cell lines using STAR-Fusion software. Four RNA-seq libraries were pooled for each cell line for a total of 12 libraries, and sequenced on a HiSeq 4000 lane. Only the K562 cell line should contain the BCR—ABL1 translocation, however reads containing BCR—ABL1 (blue and black striped) were also found in data files for the other two cell lines due to index swapping

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