Fig. 4

Multiple sequence alignment of P-type II Ca²⁺ATPases of T. aestivum. Figure shows alignment of amino acid sequences of type IIA and type IIB Ca²⁺ATPase proteins performed by multalin. The nine characteristic motifs (purple box); other reported conserved motifs (black box); the CaATP_NAI (calmodulin binding N-terminal auto inhibitory domain); the CaM binding sites (green colour) are highlighted in the figure. The presence of characteristic motifs and other conserved regions established their identity as P-type II Ca²⁺ATPases in T. aestivum