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Table 2 List of analysed FT candidate genes, sequence length, number of specific PCR fragments and detected mutations

From: Association genetics studies on frost tolerance in wheat (Triticum aestivum L.) reveal new highly conserved amino acid substitutions in CBF-A3, CBF-A15, VRN3 and PPD1 genes

Gene

Chr. position

Number of specific PCR fragments

Sequence length in bp

CDS length in bp

Gene length in bp

CDS/gene length ratio

Detected mutations

Number of polymorphic sites

CBF-D1

5D

1

709

639

639

1.00

no

0

CBF-A3

5A

1

790

741

741

1.00

yes

4

CBF-A5

7A

1

1027

633

633

1.00

yes

6

CBF-A10

5A

1

867

720

720

1.00

yes

2

CBF-A13

5A

1

855

720

720

1.00

yes

6

CBF-A14

5A

2

610/748

639

639

1.00

yes

6

CBF-A15

5A

1

786

726

726

1.00

yes

7

CBF-A18

6A

1

1045

738

738

1.00

yes

37

Dhn1

5D

1

936

420

638

0.66

no

0

VRN-A1

5A

4

1039/1097/621/770

735

11,414

0.06

yes

15

VRN-B1

5B

5

600/817/662/467/1077

735

5498

0.13

yes

24

VRN-D1

5D

4

814/1359/1429/705

735

11,550

0.06

yes

2

VRN-D2

4D

2

976/534

639

1650

0.39

no

0

VRN-B3

7B

2

1602/994

534

1258

0.42

yes

1

Cab

5A

1

728

n.a.

n.a.

n.a.

yes

28

Dem

6B/6D

1

616

n.a.

n.a.

n.a.

no

0

Tacr7

2B

1

765

n.a.

n.a.

n.a.

yes

3

PPD-B1

2B

6

1600/954/927/571/773/378

1995

3053

0.65

yes

5

PPD-D1

2D

3

726/1094/966

1983

3141

0.63

yes

109

total

 

39

34,034

13,332

43,758

0.30

 

255

  1. Chr. chromosome, bp base pairs, CDS coding DNA sequence, n.a. not available