Fig. 1

Network Analysis of the GSEA Results in the Frontal Cortex. The network shows significantly (nominal p < 0.05, false discovery rate < 0.25) enriched gene ontology (GO) terms in the gene set enrichment (GSEA) analysis in the frontal cortex (FC) of Dark Agouti rats 3 weeks after a single-dose (15 mg/kg, intraperitoneal) 3,4-methylenedioxymethamphetamine (MDMA) administration. Blue and red circles represent down- and upregulation of the associated GO terms, respectively. The size of the nodes is proportional with the number of genes in the GO term and the thickness of the grey edges represents the number of common genes between two GO terms, if any. Most of the processes in the FC were downregulated. The cellular biosynthetic process and establishment of cellular localization GO terms are in central positions of two different groups while chromosome and chromatin organization, and transmembrane transport processes form the third and fourth group of tightly related, downregulated gene sets. These alterations suggest a general neurotoxic effect while the upregulation of gene sets related to synapse formation raises the possibility of a parallel running recovery process. The expression of the only immunologically related GO term was attenuated. See text for further details