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Fig. 4 | BMC Genomics

Fig. 4

From: A map of gene expression in neutrophil-like cell lines

Fig. 4

Transcriptional profiles of HL-60 and PLB-985 cells before and after differentiation and comparison to that of primary neutrophils. a Experimental design: HL-60 and PLB-985 cells were differentiated by culturing in media supplemented with 1.3% DMSO and 9% FBS or 1.3% DMSO, 2% Nutridoma and 0.5% FBS. Total RNA of cells harvested at the indicated time points was isolated. PolyA enrichment and RNA-seq were performed by Applied Biological Materials Inc. through their Total RNA Sequencing service. RNA-seq data was subjected to our analysis pipeline as described in Methods. b Heat map of Spearman correlations between transcriptional profiles of undifferentiated and differentiated HL-60 and PLB-985 cells and human and mouse primary neutrophils, organized by unbiased hierarchical clustering. Color bar indicates correlation strength. Each row/column represents an independent sample. For the cell line data, the labels d2, d4, and d6 indicate 2, 4, and 6 days of differentiation, respectively. For the Thomas et al. data, the labels s1, s2, s3, and s4 indicate sample numbers, which correspond to the sample numbers in Table 2

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