Fig. 4From: A map of gene expression in neutrophil-like cell linesTranscriptional profiles of HL-60 and PLB-985 cells before and after differentiation and comparison to that of primary neutrophils. a Experimental design: HL-60 and PLB-985 cells were differentiated by culturing in media supplemented with 1.3% DMSO and 9% FBS or 1.3% DMSO, 2% Nutridoma and 0.5% FBS. Total RNA of cells harvested at the indicated time points was isolated. PolyA enrichment and RNA-seq were performed by Applied Biological Materials Inc. through their Total RNA Sequencing service. RNA-seq data was subjected to our analysis pipeline as described in Methods. b Heat map of Spearman correlations between transcriptional profiles of undifferentiated and differentiated HL-60 and PLB-985 cells and human and mouse primary neutrophils, organized by unbiased hierarchical clustering. Color bar indicates correlation strength. Each row/column represents an independent sample. For the cell line data, the labels d2, d4, and d6 indicate 2, 4, and 6 days of differentiation, respectively. For the Thomas et al. data, the labels s1, s2, s3, and s4 indicate sample numbers, which correspond to the sample numbers in Table 2Back to article page