Table 3 Predicted HLA DRB1-binding neo-peptides of KRAS, EGFR, TP53, and MUC16 in lung adenocarcinoma patients in this study
Gene | Mutation | HLA | MHC affinity score(nM) | Neo-peptide | Frequency of neoantigens in 147 samples |
|---|---|---|---|---|---|
KRAS | p.G12C | DRB1*01:01 | 214.21 | VGACGVGKSALTIQL | 14.97% |
p.G12Â V | DRB1*01:02 | 81.75 | VVGAVGVGKSALTIQ | 10.20% | |
p.G12A | DRB1*12:01 | 220.77 | KLVVVGAAGVGKSAL | 2.72% | |
p.G12D | DRB1*11:03 | 280.09 | KLVVVGADGVGKSAL | 0.68% | |
p.G12F | DRB1*08:04 | 89.1 | LVVVGAWRRQECLDD | 1.36% | |
p.G12R | DRB1*11:04 | 181.75 | VVVGARGVGKSALTI | 0.68% | |
p.G12S | DRB1*12:01 | 216.22 | KLVVVGASGVGKSAL | 1.36% | |
p.G12Y | DRB1*08:04 | 89.1 | LVVVGAWRRQECLDD | 0.68% | |
MUC16 | p.A5415T | DRB1*08:03 | 491.36 | TMHHSTNTAVTNVGT | 0.68% |
p.D1142Y | DRB1*01:02 | 467.11 | PYPGSARSTWLGILS | 0.68% | |
p.D9418Y | DRB1*01:02 | 42.61 | SRGPEYVSWPSPLSV | 0.68% | |
p.E11272V | DRB1*12:01 | 72.72 | ISLVTHPAVSSSTLP | 0.68% | |
p.E14134Q | DRB1*08:03 | 201.38 | QLISLRPQKDGAATG | 0.68% | |
p.E8581D | DRB1*04:04 | 399.26 | FFSTLPDSISSSPHP | 0.68% | |
p.G13025Â V | DRB1*01:02 | 161.1 | TNLQYGGHASPWLQE | 0.68% | |
p.G13669C | DRB1*04:05 | 479.01 | KFNTTERVLQCLLRS | 0.68% | |
p.G1530Â V | DRB1*12:01 | 162.76 | GIRSLGRTVDLTTVP | 0.68% | |
p.G3326R | DRB1*01:02 | 379.94 | VSLESPTARSITRTG | 0.68% | |
p.G6740C | DRB1*04:01 | 419.34 | TIITRTCPPLGSTSQ | 0.68% | |
p.H12349N | DRB1*04:04 | 254.12 | NSLYVNGFTNQSSVS | 0.68% | |
p.H14021N | DRB1*01:03 | 422.78 | HELSQQTNGITRLGP | 0.68% | |
p.L12891I | DRB1*08:03 | 420.3 | LQGLIGPMFKNTSVG | 0.68% | |
p.L2407I | DRB1*11:04 | 376.36 | SSSPSIFSSDRPQVP | 0.68% | |
p.L8172I | DRB1*04:03 | 461.25 | GFAQITVSPETSTET | 0.68% | |
p.M3792Â T | DRB1*04:01 | 445.39 | ITSAVTPAATARSSG | 0.68% | |
p.N787Y | DRB1*12:01 | 57.97 | ATSPERVRYATSPLT | 0.68% | |
p.P1203A | DRB1*01:02 | 129.39 | TTSLTASNIPTSGAI | 0.68% | |
p.P12152H | DRB1*03:01 | 473.39 | RPDHEDLGLDRERLY | 0.68% | |
p.P242H | DRB1*12:01 | 266.64 | YSSFLDLSHKGTPNS | 0.68% | |
p.P2978fs | DRB1*01:02 | 395.63 | VPLQEQGTLDMPQRA | 0.68% | |
p.P841L | DRB1*12:01 | 26.68 | STLSLLSVSGVKTTF | 0.68% | |
p.P8502A | DRB1*11:03 | 392.32 | AESAITIETGSAGAT | 0.68% | |
p.S13403I | DRB1*12:01 | 253.18 | DPKIPGLDRERLYWK | 0.68% | |
p.S1887C | DRB1*04:01 | 315.03 | KSLCMGNSTHTSMTY | 0.68% | |
p.S3428Y | DRB1*04:01 | 396.78 | TSYWSDQTSGSDITL | 0.68% | |
p.S490Y | DRB1*01:01 | 88.64 | TTGSTYGRQSSSTAA | 0.68% | |
p.S586Y | DRB1*01:02 | 479 | TYADTLIGESTAGPT | 0.68% | |
p.S6935F | DRB1*11:04 | 66.51 | TSMSVFSETTKIKRE | 0.68% | |
p.S7304Y | DRB1*16:02 | 107.01 | MLPEIYTTRKIIKFP | 0.68% | |
p.S8560C | DRB1*13:03 | 414.19 | VEEASCVSSSLSSPA | 0.68% | |
p.T12805S | DRB1*11:03 | 420.91 | NGIKELGPYSLDRNS | 0.68% | |
p.T435Â K | DRB1*13:03 | 301.6 | EGTLNKSMTPLETSA | 0.68% | |
p.T7989R | DRB1*13:03 | 492.12 | SRLPESISSSPLPVT | 0.68% | |
p.T8159A | DRB1*01:02 | 487.68 | VSRTEVASSSRTSIS | 0.68% | |
p.V11743Â M | DRB1*01:02 | 292.47 | SPGAPEMMTSQITSS | 0.68% | |
TP53 | p.A161V | DRB1*13:02 | 344.62 | RVRAMVIYKQSQHMT | 0.68% |
p.A69fs | DRB1*08:03 | 238.65 | QLRFPSGLLAFWDSQ | 0.68% | |
p.C135F | DRB1*04:07 | 85.99 | LNKMFFQLAKTCPVQ | 0.68% | |
p.C176F | DRB1*12:01 | 140.21 | YKQSQHMTEVVRRFP | 0.68% | |
p.C176Y | DRB1*12:01 | 163.66 | YKQSQHMTEVVRRYP | 0.68% | |
p.C277F | DRB1*11:04 | 200.58 | VRVCAFPGRDRRTEE | 1.36% | |
p.D281E | DRB1*11:04 | 412.76 | VRVCACPGRERRTEE | 0.68% | |
p.D281Y | DRB1*01:02 | 286.24 | RVCACPGRYRRTEEE | 0.68% | |
p.E271K | DRB1*11:04 | 283.03 | GRNSFKVRVCACPGR | 0.68% | |
p.E285K | DRB1*11:04 | 418.37 | VRVCACPGRDRRTKE | 0.68% | |
p.F270Â V | DRB1*13:03 | 401.66 | NLLGRNSVEVRVCAC | 0.68% | |
p.G245C | DRB1*08:04 | 255.62 | NSSCMGCMNRRPILT | 0.68% | |
p.G334Â V | DRB1*03:01 | 268.4 | DGEYFTLQIRVRERF | 0.68% | |
p.M237I | DRB1*07:01 | 251.46 | DCTTIHYNYICNSSC | 0.68% | |
p.N239S | DRB1*07:01 | 246.03 | YNYMCSSSCMGGMNR | 0.68% | |
p.P152fs | DRB1*01:01 | 230.65 | PVQLWVDSTPRPAPA | 0.68% | |
p.P278H | DRB1*11:04 | 318.15 | VRVCACHGRDRRTEE | 0.68% | |
p.R158L | DRB1*12:01 | 329.17 | STPPPGTRVLAMAIY | 0.68% | |
p.R175H | DRB1*01:02 | 468.06 | MTEVVRHCPHHERCS | 1.36% | |
p.R273C | DRB1*01:02 | 494.42 | EVCVCACPGRDRRTE | 0.68% | |
p.R280I | DRB1*01:02 | 445.57 | VRVCACPGIDRRTEE | 0.68% | |
p.R337L | DRB1*01:01 | 225.82 | FTLQIRGRELFEMFR | 0.68% | |
p.S127C | DRB1*16:01 | 380.13 | VTCTYCPALNKMFCQ | 0.68% | |
p.V73Â fs | DRB1*01:02 | 15.9 | WPLHQQLLHRRPLHQ | 0.68% | |
EGFR | p.709_710ET > D | DRB1*16:02 | 411.98 | SGEAPNQALLRILKE | 1.36% |
p.773_774insH | DRB1*12:01 | 281.83 | VMASVDNPHVCRLLG | 0.68% | |
p.ELR746del | DRB1*01:02 | 169.33 | ELREATSPKANKEIL | 1.36% | |
p.ELREA746del | DRB1*01:02 | 77.85 | KELREATSPKANKEI | 0.68% | |
p.K754I | DRB1*01:03 | 230.19 | ELREATSPIANKEIL | 0.68% | |
p.L858R | DRB1*08:03 | 205.08 | ITDFGRAKLLGAEEK | 1.36% | |
p.L861Q | DRB1*09:01 | 464.7 | TDFGLAKQLGAEEKE | 0.68% | |
p.Q432H | DRB1*16:01 | 363.6 | LEIIRGRTKHHGQFS | 0.68% | |
p.S768I | DRB1*03:01 | 212.84 | AYVMAIVDNPHVCRL | 0.68% | |
p.TSPKANKE751del | DRB1*01:01 | 95.63 | IKELREATSPKANKE | 0.68% | |
p.V769Â L | DRB1*04:04 | 253.85 | VMASLDNPHVCRLLG | 0.68% |