Fig. 6

Functionally annotated Agave genes showing positive selection and diel rewiring of expression pattern relative to C₃ plants. a Genes involved in circadian clock. A gene involved in stomatal opening. c Genes involved in carboxylation, malate transport, decarboxylation, and starch/sugar metabolism. d Genes involved in photosynthetic electron transport chain. Red circles indicate positive selection. Green circles indicate morning-to-night shift in peak gene expression. Yellow circles indicate afternoon-to-night shift in peak gene expression. AGP16, Arabinogalactan protein 16; AKT2, Arabidopsis Shaker family K⁺ channels 2/3; CT-BMY, Chloroplast Beta-Amylase; ELF3, Early Flowering 3; GAUT7, Galacturonosyltransferase 7; LHY, Late Elongated Hypocotyl; LUX, Phytoclock 1; PPCK1, Phosphoenlpyruvate Carboxylase Kinase 1; PPDK-RP, Pyruvate orthophosphate dikinase regulatory protein; SS2, Starch Synthase 2; TDT, Tonoplast Dicarboxylate Transporter