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Fig. 2 | BMC Genomics

Fig. 2

From: Proteomic and evolutionary analyses of sperm activation identify uncharacterized genes in Caenorhabditis nematodes

Fig. 2

Schematic of The Shredder. a The Shredder is a microfluidic dissection device with a single worm loading arena, a needle insertion, a sperm filtration and collection arena, and two flush channels. b The male loading arena. The bifurcating design sequentially loads males into the dissection channel. c The male dissection channel. Males are pushed into the channel from the loading arena and sperm cells are flushed out the right. The needle channel is separated from the male dissection channel by a thin filament of PDMS, which creates a water-tight seal around the needle. d The sperm filter (10 um) prevents collection of non-sperm components. e Males in the loading arena for sequential loading into the dissection channel. f Dissected male and released spermatids (indicated by the triangle) for collection

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