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Fig. 9 | BMC Genomics

Fig. 9

From: Deciphering the oviductal extracellular vesicles content across the estrous cycle: implications for the gametes-oviduct interactions and the environment of the potential embryo

Fig. 9

Validation of Mass Spectrometry and miRNA analyses of bovine oEVs by Western immunoblotting and qPCR. a Western blot analysis confirmed MS results for 3 proteins associated with reproductive roles and showing differential abundance or not across the bovine estrus cycle: CD109, HSPA8 and MYH9. A pool of samples containing 3 biological replicates at the 4 different stages analyzed was used. b qPCR analysis confirmed miRNA-seq results for Mir-449a, which dysregulation has been associated with defective cilia in the oviduct and infertility. Mir-449a was more abundant in S1 compared to the rest of the stages (p < 0.05, T-test). A new batch of oEVs isolated from oviducts classified in the four stages of the estrous cycle (n = 12) was used. c Network of Mir-449a target genes and overrepresented functional categories

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BMC Genomics

ISSN: 1471-2164

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