Fig. 3From: Iron-dependent essential genes in Salmonella TyphimuriumIllustration of the iron-independent essential and iron-dependent essential genes as identified by Tn-seq. A genome-saturating library S. Typhimurium Tn5 mutants was inoculated to iron-replete (LB medium) and iron-restricted conditions (LB medium supplemented with an iron chelator, 2,2`-Dipyridyl). The cultures were grown till mid-log phase and then subjected to Tn-seq to identify essential genes under each of the two conditions. Examples of iron-independent essential gene (accA), non-essential gene (rpoS) and iron-dependent essential gene (ftsI) are shown. For accA gene, no insertion mutant was recovered from both conditions. For ftsI gene, on the contrary, insertion mutants were recovered in iron-restricted condition, but not in iron-replete conditionBack to article page