Fig. 3

Comparative ORC2 binding analysis across Drosophila tissues and cell culture lines. a Comparison of high-confidence ORC2 binding sites (Irreproducible Discovery Rate, IDR < 0.1) from 2nd-instar larval fat body (FB), mid-3rd-instar larval FB, late-3rd-instar larval FB, late-3rd-instar larval salivary gland (SG) [8], Kc cells, S2 cells, and BG3 cells [6]). ORC2 binding sites within 1 kb were treated as overlapping. b Comparison of 2nd-instar FB ORC2 sites with late-3rd-instar larval SG, Kc cells, S2 cells, and BG3 cells. 2nd-instar larval FB specific ORC sites (black), 2nd-instar larval FB sites shared with one (red), two (green), three (blue), or all other cell and tissue types (orange) are depicted. c Rectangular Venn diagram comparing the relationship between ORC binding and transcription start sites (TSSs) in 2nd-instar larval FB. All transcription start sites within 1 kb of an ORC site were identified, and the percentile rank of the corresponding transcript (in FPKM) was determined from FB and from Kc cell RNA-seq data [46]. The difference in percentile rank (DPR) was then calculated for each transcript between FB and Kc cells. Each transcript was then classified as FB-specific (FB>> Kc, for DPR > 40, black), higher in FB or no difference (FB ≥ Kc, for DPR between 1 and 40, blue), lower in FB or no difference (FB ≤ Kc, for DPR ≤ 0, yellow), or not expressed in FB (N.E., white)