In vivo evolution of drug-resistant Mycobacterium tuberculosis in patients during long-term treatment

Xu, Yuhui; Liu, Fei; Chen, Suting; Wu, Jiannan; Hu, Yongfei; Zhu, Baoli; Sun, Zhaogang

doi:10.1186/s12864-018-5010-5

BMC Genomics

Table 2 Medication and drug resistance of the phenotypes and genotypes of four refractory tuberculosis patients

From: In vivo evolution of drug-resistant Mycobacterium tuberculosis in patients during long-term treatment

		Patient A^#					Patient B			Patient C					Patient D
Drug	gene^a	A1^#	A2	A3	A4	A5	B1	B2	B3	C1	C2	C3	C4	C5	D1	D2	D3	D4	D5
H	katGa	T326 M	T326 M	T326 M	T326 M	T326 M	Y98S	Y98S	Y98S	S315 T	S315 T	S315 T	S315 T	S315 T	S315 T	S315 T	S315 T	S315 T	S315 T
H	inhA promotor	−15, C-T	−15, C-T	− 15,C-T	−15, C-T	− 15,C-T				−15, C-T	−15, C-T	− 15, C-T	−15, C-T	− 15, C-T
R	rpoB	H526L	H526L	H526L	H526L	H526L	H526D	H526D	H526D	D516V	D516V	N373D/ D516V	N373D/ D516V	N373D/ D516V	S531 L	S531 L	S531 L	S531 L	S531 L
Z	pncA	A87T	A87T	A87T	A87T	A87T	A87T	A87T	A87T	A87T	A87T	A87T	A87T	A87T	A87T	A87T/ L120P	A87T/ L120P	A87T/ L120P	A87T/ L120P
E	embB	M306 V	M306 V	M306 V	M306 V	M306 V	Y319S	Y319S	Y319S	G406A	G406A	G406A	G406A	G406A	M306 V	M306 V	M306 V	M306 V	M306 V
	Rv3806c							A249G	A249G						F140 V	F140 V	F140 V	F140 V	F140 V
	Rv3756c (proZ)	G56D	G56D	G56D	G56D	G56D
	Rv1699 (pyrG)				G407D	G407D
S	rpsL	K88R	K88R	K88R	K88R	K88R										K43R	K43R	K43R	K43R
S	rrs						c1402t/ a1482g	c1402t/ a1482g	c1402t/ a1482g	a514c c1402t	a514c c1402t	a514c c1402t	a514c c1402t	a514c c1402t	a1401g	a1401g	a1401g	a1401g	a1401g
LFX	gyrA	D94G	D94G	D94G	D94G	D94G	A90V	A90V	A90V	A90V	A90V/ S91P	A90V/ S91P	A90V/ S91P	A90V/ S91P		D94N	D94N/ A90V	D94N/ A90V	D94N/ A90V
PAS	Rv2447c (folC)		I43T	I43T	I43T	I43T	E153G	E153G	E153G
Amk	Rv2416c (eis)				P2L	P2L
MDR+	Rv1129c	P131T	P131T	P131T	P131T	P131T	P131T	P131T	P131T	D160A	D160A	D160A	D160A	D160A	P131T	P131T	P131T	P131T	P131T
CPM/Amk	Rv0323c		V215 L	V215 L	V215 L	V215 L	V215 L	V215 L	V215 L	V215 L	V215 L	V215 L	V215 L	V215 L	V215 L	V215 L	V215 L	V215 L	V215 L
LFX/ETH	Rv0404 (fadD30)							L10R	L10R	L10R	L10R	L10R	L10R	L10R	L10R	L10R	L10R	L10R	L10R
CPM/Amk/ LFX/ETH	Rv0565c						F68I	F68I	F68I							T402 M	T402 M	T402 M	T402 M
ETH + Amk	Rv2080 (lppJ)						S82^a	S82^a	S82^a	S82^a	S82^a	S82^a	S82^a	S82^a
ETH + Amk + CMP + S	Rv2447c (folC)		I43T	I43T	I43T	I43T	E153G	E153G	E153G
Drugs ever used		H R Z E	E Pa LFX PAS Amk	E Clr Pa LFX PAS Amk	E Clr Pa LFX PAS Amk	E Clr Pa LFX PAS Amk	R Z CPM LFX ETH Clr	R Z CPM LFX ETH Clr	E Clr PAS MFX Rfb	E Amk PAS ETH LFX	E Amk PAS ETH LFX	E Amk PAS ETH LFX	E Amk PZA PAS ETH LFX	E Amk PZA PAS ETH LFX	E Z Amk Clr CPM LFX	E Z Amk Clr CPM LFX	E Z S Amk Clr CFZ MFX	E Z S Amk Clr CFZ MFX	E Z S Amk Clr CFZ MFX
Resistant to		H R S LFX ETH	H R S LFX ETH PAS	H R S LFX ETH PAS	H R S LFX Amk ETH PAS	H R S LFX Amk ETH PAS	H R LFX ETH PAS	H R LFX ETH PAS CPM	H R E S LFX ETH PAS CPM	H R CPM	H R Lfx CPM	H R Lfx CPM	H R Lfx CPM	H R S Lfx CPM	H R LFX ETH PAS	H R LFX Amk ETH PAS	H R LFX Amk ETH PAS	H R S LFX Amk ETH PAS	H R S E Amk LFX ETH PAS CPM

H isoniazid, R rifampin, S streptomycin, Z pyrazinamide, E ethambutol, LFX levofloxacin, MFX moxifloxacin, PAS p-aminosalicylic acid sodium, Amk amikacin, ETH ethionamide, CPM capreomycin, Clr clarithromycin, CFZ Clofazimine
^adrug-resistant related genes mainly based on the report by Zhang et al. [5]
^#A, B, C, and D indicate the four cases, and the number following them indicates the different isolates

Back to article page

ISSN: 1471-2164

Contact us

Submission enquiries: bmcgenomics@biomedcentral.com
General enquiries: ORSupport@springernature.com