Fig. 2

HOXC13 had a similar expression pattern with the majority of KRTs in anagen and telogen. a Differentially expressed KRTs and related regulatory genes were shown in Heat Map. HOXC13 had similar expression trend with the majority of KRTs, which were markedly up-regulated in anagen compared with telogen. b qRT-PCR was performed on selected KRTs, HOXC13 and DSG4 to verify the sequencing data. HOXC13, DSG4, KRT38 and KRT84 were up-regulated, while KRT1 and KRT2 were down-regulated in anagen compared with telogen. The expression of specific gene was quantified relative to the expression level of β-actin using the comparative cycle threshold (ΔCT) method. The data was expressed as the mean ± 1 SE (n = 3). * P < 0.05. c Immunostaining of HOXC13 in cashmere goat hair follicles at embryonic period (65d and 120d), anagen and telogen. Red fluorescence indicated the nuclear expression pattern of HOXC13. Nucleus was stained with Hoechst in blue