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Fig. 4 | BMC Genomics

Fig. 4

From: The inconsistent regulation of HOXC13 on different keratins and the regulation mechanism on HOXC13 in cashmere goat (Capra hircus)

Fig. 4

HOXC13 had an inconsistent regulation on the promoters of KRT1 and KRT2 compared with KRT84 and KRT38. a-d The regulation of HOXC13 on keratin promoters including KRT1, KRT2, KRT38 and KRT84 were detected. The corresponding KRT promoter-pGL3 basic vector, pRL-TK vector together with HOXC13 overexpression vector (wtHOXC13/ΔHOXC13) were co-transfected into 12-well dermal papilla cells, while the vector of ΔHOXC13 was used as a negative control. * P < 0.05, **P < 0.01 (e-f) The regulation function of HOXC13 on keratin promoters including KRT2 and KRT84 were detected using KRT-promoter-GFP system. In this system, the CMV promoter before GFP was taken place by the promoter of KRT. The corresponding KRT promoter-GFP vector together with HOXC13 overexpression vector (wtHOXC13/ΔHOXC13) were co-transfected into 12-well HEK 293 T cells while the vector of ΔHOXC13 was used as a negative control. All the groups were performed in triplicate. The data was expressed as the mean ± 1 SE (n = 3). * P < 0.05, ** P < 0.01

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