Characteristic | Sabino et al. [5] | Diab-Elschahawi et al. [6] | Reiss et al. [7] | Vaz et al. [8] | Trobajo-Sanmartín et al. (This study) |
---|---|---|---|---|---|
PCR scheme used | Singleplexb | Multiplex | Multiplex | Multiplex | Multiplex |
Redesign of the original primers | NA | Yes | Yes | Yes | No |
Number of primers pairs used for each PCR reaction | 1 | 3 | 1 | 4 | 4 |
Total number of PCR reactions needed for a complete STR analysis | 3 | 2 | 5 | 1 | 1 |
Number of dyes used in each genotyping reaction | 3 | 3 | 1 | 3 | 4 |
Evaluation of the sensitivity and specificitya | NA | No | No | No | Yes |
Evaluation of the microsatellite informativeness parameters | Yes | Unknownc | Unknownc | Unknownb | Yes |
Total time elapsed to obtained results (in minutes) | 138 | 79 | 197 | 138 | 79 |
Approximate costd estimated of the primer pairs used in each protocol (in euros) | 5,60 | 15,72 | 7,00 | 9,26 | 12,76 |
Approximate total costd estimated for the complete STR analysis of one sample (in euros) | 15,58 | 22,53 | 20,77 | 14,00 | 17,45 |