Table 5 Direct concordance and Kappa indices of the multiplex PCR touchdown protocol
Multiplex PCR approach genotyping technique (this study) | Original genotyping technique [5] | ||||||
Clade A1 | Clade A2 | Clade B1 | Clade B2 | Clade C1 | Clade C2 | Total | |
Clade A1 | 3 | 0 | 0 | 0 | 0 | 0 | 3 |
Clade A2 | 1 | 5 | 0 | 0 | 0 | 0 | 6 |
Clade B1 | 0 | 0 | 8 | 0 | 0 | 0 | 8 |
Clade B2 | 0 | 0 | 0 | 0 | 0 | 1 | 1 |
Clade C1 | 0 | 0 | 0 | 0 | 12 | 0 | 12 |
Clade C2 | 0 | 0 | 0 | 0 | 0 | 3 | 3 |
Total | 4 | 5 | 8 | 0 | 12 | 4 | 33 |
Agreement | Expected agreement | Kappa index | Std. Error of Kappa index | Z | p-value | ||
0.939 | 0.240 | 0.920 | 0.0917 | 10.04 | < 0.001 | ||